摘要

在Fildes Peninsula（King George Island，South）从不同的地点分离出总共63个耐受各种抗生素（如氨苄青霉素，链霉素和/或甲氧苄啶）的耐冷细菌，这些不同的地点在人类影响方面有所不同，从明显存在到可能不存在设得兰群岛）。进一步确定了这些抗生素抗性分离株中一类整合子的存在。将来自两个分离株（HP19和CN11）的质粒通过缀合转移至大肠杆菌DH5α。来自HP19分离株的质粒的序列分析与肠杆菌hormaechei亚种的质粒p34998-210.894kb具有高度相似性（约99％）。 steigerwaltii的临床起源，并证实在1类整合子背景下存在dfrA14盒。这些耐冷菌分离物中的5个的16S rRNA基因序列分析表明与先前鉴定为肠杆菌属物种的环境细菌相似。总之，这些结果证实，抗生素抗性基因传播可能没有原始的利基。

A total of 63 psychrotolerant bacteria exhibiting resistance to various antibiotics, such as ampicillin, streptomycin and/or trimethoprim, were isolated from diverse sites varying in terms of human influence, from obvious presence to probable absence, on Fildes Peninsula (King George Island, South Shetland Islands). The presence of class 1 integrons in some of these antibiotic resistant isolates was further determined. Plasmids from two isolates (HP19 and CN11) were transferred to Escherichia coli DH5α by conjugation. Sequence analysis of the plasmid from the HP19 isolate exhibited high similarity (~99%) to plasmid p34998-210.894kb of Enterobacter hormaechei subsp. steigerwaltii of clinical origin and confirmed the presence of a dfrA14 cassette in a class 1 integron context. 16S rRNA gene sequence analysis of five of these psychrotolerant isolates indicated similarity with environmental bacteria previously identified asEnterobacter species. Together, these results confirm that there may be no pristine niches for antibiotic resistance gene dissemination.

https://www.cambridge.org/core/journals/antarctic-science/article/bacteria-from-fildes-peninsula-carry-class-1-integrons-and-antibiotic-resistance-genes-in-conjugative-plasmids/37B5662DE83711365D3AF9E5A5C16C4A