摘要
来自污水污泥的抗生素抗性基因（ARG）的传播被强调为突出的健康威胁。这项研究建立了嗜热厌氧消化器，采用一步启动策略，快速从污泥中去除四环素和磺胺抗药性基因。在嗜中性至嗜热状态的启动阶段至少节省20天。基于16S rDNA扩增子测序和预测的宏基因组学方法的结果，成功启动很大程度上依赖于核心嗜热微生物群体（例如厚壁菌门，变形杆菌，放线菌）的快速定植。基质降解和甲烷产生的微生物代谢基因途径也通过一步模式增加。此外，实时定量PCR方法显示，在嗜热消化（去除效率> 80％）期间，大部分靶向的四环素和磺酰胺抗性基因ARG（sulI，tetA，tetO，tetX）被基本去除。网络分析表明，ARGs的消除归因于它们在高温下的水平（intI1项目）和垂直（潜在寄主）转移相关元件的下降。这项研究表明，废水固体的快速启动嗜热厌氧消化将是减少各种ARGs数量的合适技术。
Spread of antibiotic resistance genes (ARGs) originating from sewage sludge is highlighted as an eminent health threat. This study established a thermophilic anaerobic digester using one-step startup strategy to quickly remove tetracycline and sulfonamides resistance genes from sewage sludge. At least 20 days were saved in the startup period from mesophilic to thermophilic condition. Based on the results of 16S rDNA amplicons sequencing and predicted metagenomic method, the successful startup largely relied on the fast colonization of core thermophilic microbial population (e.g. Firmicutes,Proteobacteria, Actinobacteria). Microbial metabolic gene pathways for substrate degradation and methane production was also increased by one-step mode. In addition, real-time quantitative PCR approach revealed that most targeted tetracycline and sulfonamides resistance genes ARGs (sulI, tetA, tetO, tetX) were substantially removed during thermophilic digestion (removal efficiency > 80%). Network analysis showed that the elimination of ARGs was attributed to the decline of their horizontal (intI1 item) and vertical (potential hosts) transfer-related elements under high-temperature. This research demonstrated that rapid startup thermophilic anaerobic digestion of wastewater solids would be a suitable technology for reducing quantities of various ARGs.
https://www.sciencedirect.com/science/article/pii/S0048969717323173
