摘要
      含有兽用抗生素（VA）和重金属的土壤需要有效的修复方法，应进一步检查微生物和分子水平的结果。本文建立了一种结合废菌糠基生物炭（WFCB）和胡天草螺菌（HHS1）的新型材料，用于固定铜（Cu）和锌（Zn），降解土霉素（OTC）和恩诺沙星（ENR）。结果表明，该复合材料对Cu（85.5%）和Zn（64.4%）具有很高的固定化作用，对OTC（41.9%）和ENR（40.7%）具有很好的去除作用。包括tet（PB）、tetH、tetR、tetS、tetT、tetM、aacA/aphD、aacC、aadA9和czcA在内的抗性基因被降低。抗生素抗性基因（ARGs）的潜在宿主的丰度发生了变化，包括变形菌门、短杆菌属和红肠杆菌属。总磷和pH是驱动VA降解微生物和ARGs潜在宿主的因素。WFCB和HHS1的组合可以作为一种重要的生物源，用于固定重金属和去除污染土壤中的VA。
Abstract
Soils containing both veterinary antibiotics (VAs) and heavy metals necessitate effective remediation approaches, and microbial and molecular levels of the results should be further examined. Here, a novel material combining waste fungus chaff-based biochar (WFCB) and Herbaspirillum huttiense (HHS1) was established to immobilize copper (Cu) and zinc (Zn) and degrade oxytetracycline (OTC) and enrofloxacin (ENR). Results showed that the combined material exhibited high immobilization of Cu (85.5%) and Zn (64.4%) and great removals of OTC (41.9%) and ENR (40.7%). Resistance genes including tet(PB), tetH, tetR, tetS, tetT, tetM, aacA/aphD, aacC, aadA9, and czcA were reduced. Abundances of potential hosts of antibiotic resistance genes (ARGs) including phylum Proteobacteria and genera Brevundimonas and Rhodanobacter were altered. Total phosphorus and pH were the factors driving the VA degrading microorganisms and potential hosts of ARGs. The combination of WFCB and HHS1 can serve as an important bioresource for immobilizing heavy metals and removing VAs in the contaminated soil.

https://www.sciencedirect.com/science/article/abs/pii/S0045653522008268