摘要

我们调查了114种沙门氏菌属菌株的遗传相关性，抗生素抗性和生物膜生产能力，属于三种血清型（Corvallis，Brancaster和Albany），它们是从天然污染的家禽及其在潮湿的市场环境中分离出来的，并且来自北方的小规模加工厂马来西亚。脉冲场凝胶电泳显示从不同市场分离的沙门氏菌菌株与克隆相关，表明这三种血清型在马来西亚北部广泛传播。除一种沙门氏菌外，其他所有沙门氏菌均对两种以上抗生素产生耐药性，因此被视为多药耐药性（MDR）。对磺酰胺（96.5％），氨苄青霉素（89.5％），四环素（85.1％），氯霉素（75.4％），甲氧苄啶（68.4％），甲氧苄啶磺胺甲恶唑（67.5％），链霉素（58.8％）和萘啶酮酸（44.4％ ％）被观察到。通过PCR检测MDR沙门氏菌菌株中的耐药决定子，floR，cmlA，tetA，tetB，tetG，temB，blaPSE-1，sul1，sul2，qnrA，qnrS，strA和aadA。 76株（66.7％）含有I类整合子。鉴定的基因盒是dfrA1，dfrA12，aadA2和功能未知的开放阅读框orfC。所有沙门氏菌菌株都产生生物膜，其中69.3％是强生物膜生产者。我们的研究结果表明，最有可能的持续性沙门氏菌通过生产生物膜在加工环境中的不同部位定殖，这导致其在位于马来西亚北部的湿市场中广泛传播。

We investigated the genetic relatedness, antibiotic resistance and biofilm-producing ability of 114 strains of Salmonella, belonged to three serotypes (Corvallis, Brancaster and Albany), isolated from naturally contaminated poultry and their environment in wet markets and smale-scale processing plant from northern Malaysia. Pulsed-field gel electrophoresis revealed that Salmonella strains isolated from various wet markets were clonally related, suggesting the widespread dissemination of these three serotypes in northern Malaysia. All except one strain of Salmonella were resistant to more than two classes of antibiotics, hence regarded as multidrug resistant (MDR). Resistance to sulphonamide (96.5%), ampicillin (89.5%), tetracycline (85.1%), chloramphenicol (75.4%), trimethoprim (68.4%), trimethoprim-sulfamethoxazole (67.5%), streptomycin (58.8%) and nalidixic acid (44.4%) were observed. Resistance determinants, floR, cmlA,tetA, tetB, tetG, temB, blaPSE-1, sul1, sul2, qnrA, qnrS, strA and aadA were detected byPCR among MDR Salmonella strains. Seventy-six strains (66.7%) harboured class-I integrons. The gene cassettes identified were dfrA1, dfrA12, aadA2 and an open reading frame orfC with unknown function. All Salmonella strains produced biofilm and 69.3% of them were strong biofilm-producers. Our findings suggested that most likely, persistentSalmonella colonises various sites in the processing environment by producing biofilm, which leads to their widespread dissemination in wet markets located in northern Malaysia.

https://www.sciencedirect.com/science/article/pii/S0963996917308360