摘要
     本文利用生物化学甲烷电位（BMP）试验研究了家禽和牛粪的中温（35°C）和嗜热（55°C）生物甲烷化。分析了比甲烷产量（SMP）、24种药物化合物（PhAC）和5种抗生素抗性基因（ARGs）（blaKPC、ermB、qnrS、sul1和tetW）以及微生物群落。当使用家禽粪便时，嗜中温BMP测试导致最高的SMP（家禽为285.5 mL CH4/g VSS，牛粪为239.6 mL CH4/gVSS），而嗜热温度导致牛粪为最高的SMP。在55°C下检测到兽药的去除率更高，两种肥料都表明嗜热消化更适合去除这些化合物。泰乐菌素、替米考星、金霉素和磺胺甲恶唑的去除率高于50%，是在中温和高温条件下完全去除的前两种。当比较每次处理结束时ARGs的相对丰度时，发现qnrS的去除最显著，而在厌氧处理后没有检测到。其余的ARG没有发生重大变化。最后，微生物组成分析表明，温度对最终微生物种群的影响大于基质或接种物中存在的微生物。
Abstract
In the present paper, the mesophilic (35 °C) and thermophilic (55 °C) biomethanization of poultry and cattle manures were investigated using biochemical methane potential (BMP) tests. Specific methane production (SMP), 24 pharmaceutical compounds (PhACs), and five antibiotic resistance genes (ARGs) (blaKPC, ermB, qnrS, sul1 and tetW) together with the microbial community were analyzed. Mesophilic BMP tests resulted in the highest SMP when poultry manure was used (285.5 mL CH4/g VSS with poultry vs 239.6 mL CH4/g VSS with cattle manure) while thermophilic temperatures led to the highest SMP with cattle manure (231.2 mL CH4/g VSS with poultry vs 238.0 mL CH4/g VSS with cattle manure). Higher removals of veterinary pharmaceuticals were detected at 55 °C with both manures indicating that thermophilic digestion is better suited for the removal of these compounds. Tylosin, tilmicosin, chlortetracycline, and sulfamethoxazole presented removals higher than 50%, being the first two completely removed under mesophilic and thermophilic conditions. When comparing the relative abundance of ARGs at the end of each treatment, the most significant removal was found for qnrS which was not detected after the anaerobic treatment. The remaining ARGs did not suffer significant changes. Finally, microbial composition analysis showed that temperature affected the final microbial population more than the microorganisms present in the substrate or inoculum.

https://www.sciencedirect.com/science/article/abs/pii/S0048969722005691