摘要

通过基质辅助激光解吸/电离飞行时间质谱法开发了二氧化钛改性靶板以增强完整细菌分析。该板设计用于光催化破坏细菌包膜结构并提高细胞内成分的电离效率，从而促进可测量的质量范围和可实现的检测灵敏度。因此，通过完整细菌的质谱指纹图谱建立了用于快速检测抗微生物剂抗性相关蛋白质的方法，其赋予细菌对抗微生物药物的抗性，而不需要任何样品预处理。用这种方法，可从相对峰强度快速测量细菌内抗性蛋白质表达水平的变化。通过质谱法直接从完整细菌中检测抗性蛋白质的这种方法可用于在进行物种鉴定的同时快速区分耐抗微生物细菌与它们的非抗性对应物。此外，它可以作为初步确定潜在的耐药机制的快速和方便的方式。

Titanium dioxide-modified target plates were developed to enhance intact bacteria analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The plates were designed to photocatalytically destroy the bacterial envelope structure and improve the ionization efficiency of intracellular components, thereby promoting the measurable mass range and the achievable detection sensitivity. Accordingly, a method for rapid detection of antimicrobial resistance-associated proteins, conferring bacterial resistance against antimicrobial drugs, was established by mass spectrometric fingerprinting of intact bacteria without the need for any sample pre-treatment. With this method, the variations in resistance proteins’ expression levels within bacteria were quickly measured from the relative peak intensities. This approach of resistance protein detection directly from intact bacteria by mass spectrometry is useful for fast discrimination of antimicrobial-resistant bacteria from their non-resistant counterparts whilst performing species identification. Also, it could be used as a rapid and convenient way for initial determination of the underlying resistance mechanisms.

http://pubs.rsc.org/en/content/articlehtml/2018/sc/c7sc04089j