摘要

从沉积物中分离出89种培养的假单胞菌属物种，并从受石油和工业影响的五个红海工业区采集水样。基因型（exoT，exoS，exoU，exoY，lasA，lasB，rhlA，rhlB，Pf1，PAGI-1，-2和-3）和表型（DNA酶，弹性蛋白酶，脂肪酶，蛋白酶，铁载体，抗生素耐药型）决心。在这些分离株中，选择了9株假单胞菌作为高活性毒力因子生产者，同时对本研究中包含的所有不同类别的抗生素具有高度抗性模式。通过16S rRNA基因扩增和测序对它们进行表型和化学表征以及分子鉴定。通过乙酸乙酯提取这9种菌株的生物活性代谢物，然后评估它们对大鼠肝组织，肾组织和其他生物化学活性的细胞毒活性。 EGY6和EGY8导致压缩细胞体积（PCV），红细胞计数（RBC）和血红蛋白（Hb）水平显着降低，这表明这些假单胞菌菌株代谢物可引起贫血和对血液学值的毒性作用在被它们感染的动物中。用毒性最强的提取物EGY8处理的大鼠在肝脏和肾脏中显示出严重的组织病理学改变。

Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, siderophore, antibiotic resistance patterns) characteristics were determined. Out of these isolates, nine Pseudomonas isolates were selected as the hyperactive virulence factors producers along with highly resistant pattern against all antibiotics of different classes included in this study. They were subjected to phenotypic and chemotypic characterization as well as molecular identification through 16S rRNA gene amplification and sequencing. The bioactive metabolites of these nine strains were extracted by ethyl acetate followed by evaluating their cytotoxic activity toward liver tissues, kidney tissues, and other biochemical activities in rat. Both EGY6 and EGY8 caused the highest significant reduction in the levels of packed cell volume (PCV), red blood cell count (RBC), and hemoglobin (Hb), which indicate that these Pseudomonas strain metabolites could cause anemia and toxic effects on hematological values in animals that were infected with them. Rats treated with the most toxic extract, EGY8, showed severe histopathological alterations in liver and kidney.

https://link.springer.com/article/10.1007/s12010-017-2553-x