摘要

立枯丝核菌是一种植物病原真菌，可感染包括水稻在内的各种经济作物。在这种情况下，这种病原体的生物防治是有效控制病原体的基本途径之一。 Pseudomonas parafulva PRS09-11288株是从水稻根际中分离得到的，并显示出对抗R. solani的生物防治能力。在这里，我们分析了P. parafulva基因组，这是〜4.7 Mb，与4310编码序列，76 tRNAs和7 rRNAs。基因组分析鉴定了一种吩嗪生物合成途径，它可以产生抗生素吩嗪-1-羧酸（PCA）。该化合物负责对水稻中最严重的真菌病之一的水稻锈病菌（R.solaniKühn）的生防能力。在此途径中非常重要的吩嗪生物合成基因突变体ΔphzF的分析证实了使用LC-MS谱的途径和PCA产生之间的关系。该菌株注释的全基因组序列揭示了P.parafulva PRS09-11288作为生物防治细菌的作用。

       Rhizoctonia solani is a plant pathogenic fungus, which can infect a wide range of economic crops including rice. In this case, biological control of this pathogen is one of the fundmental way to effectively control this pathogen. The Pseudomonas parafulva strain PRS09-11288 was isolated from rice rhizosphere and shows biocontrol ability against R. solani. Here, we analyzed the P. parafulva genome, which is ~ 4.7 Mb, with 4310 coding sequences, 76 tRNAs, and 7 rRNAs. Genome analysis identified a phenazine biosynthetic pathway, which can produce antibiotic phenazine-1-carboxylic acid (PCA). This compound is responsible for biocontrol ability against R. solani Kühn, which is one of the most serious fungus disease on rice. Analysis of the phenazine biosynthesis gene mutant, ΔphzF, which is very important in this pathway, confirmed the relationship between the pathway and PCA production using LC-MS profiles. The annotated full genome sequence of this strain sheds light on the role of P. parafulvaPRS09-11288 as a biocontrol bacterium.

https://link.springer.com/article/10.1007/s00284-018-1441-0