摘要

抗微生物药物耐药性的持续威胁对研究人员开发确保细菌感染仍可治愈的新策略提出了相当大的挑战。已显示许多植物提取物具有抗菌特性，并可能与其他抗菌剂组合以产生更有效的制剂。在这项研究中，三种植物提取物和毒性噬菌体的抗菌活性已经作为单独组分和组合进行了评估。当用改进的悬浮试验进行评估时，这些植物提取物在细菌抑制浓度下也表现出抗病毒活性。因此，为了研究提取物和毒性噬菌体之间的任何潜在的累加效应，提取物在亚抗病毒浓度下进行测试。与未经处理和提取物处理的对照相比，单独的噬菌体和植物提取物组合物显着降低了细菌浓度（P <0.05）达6小时（2-3log10），但这种减少不延长至24小时。在大多数情况下，与单独的噬菌体相比，噬菌体和提取物组合没有显着降低细菌含量。此外，噬菌体在其细菌宿主内繁殖的能力几乎没有影响。据我们所知，这项研究是首次将抗微生物植物提取物与毒性噬菌体结合，并强调植物提取物在设计组合疗法之前必须进行功能性鉴定。

The continuing threat of antimicrobial resistance presents a considerable challenge to researchers to develop novel strategies ensuring that bacterial infections remain treatable. Many plant extracts have been shown to have antibacterial properties and could potentially be combined with other antibacterial agents to create more effective formulations. In this study, the antibacterial activity of three plant extracts and virulent bacteriophages have been assessed as individual components and in combination. When assessed with a modified suspension test, these plant extracts also exhibit antiviral activity at bacterial inhibitory concentrations. Hence, to investigate any potential additive effects between the extracts and virulent phages, the extracts were tested at subantiviral concentrations. Phages alone and in combination with plant extracts significantly reduced (P < 0·05) the bacterial concentration compared to untreated and extract treated controls up to 6 h (2–3log10), but this reduction did not extend to 24 h. In most cases, the phage and extract combinations did not significantly reduce bacterial content compared to phages alone. Additionally, there was little impact on the ability of the phages to reproduce within their bacterial hosts. To our knowledge, this study represents the first of its kind, in which antimicrobial plant extracts have been combined with virulent phages and has highlighted the necessity for plant extracts to be functionally characterized prior to the design of combinatorial therapies.

https://onlinelibrary.wiley.com/doi/abs/10.1111/lam.12838