摘要
      抗生素抗性细菌（ARB）和抗生素抗性基因（ARGs）通过质粒介导的偶联传播引起了人们的广泛关注。在本研究中，研究了硫化纳米级零价铁（S-nZVI）/过氧一硫酸盐（PMS）和S-nZVI/过氧二硫酸盐（PDS）工艺灭活ARB（带有RP4质粒的大肠杆菌DH5α，假单胞菌。HLS-6在基因组DNA序列上含有sul1和intI1）。S-nZVI/PMS系统在ARB失活方面表现出比S-nZVII/PDS更高的效率。因此，最佳条件28 mg/L S-nZVI与153.7偶联 mg/L（0.5 mM）PMS来去除细胞内ARG（iARGs）和ARB。通过细胞活力、细胞内Mg2+水平、细胞外和内部结构的变化、细胞壁和膜的完整性以及酶活性，系统地研究了ARB细胞的氧化损伤。S-nZVI/PMS有效灭活ARB（~7.32 日志）在15内 分钟。这些效果大大高于单独实现的效果。此外，iARGs sul1、intI1和tetA的去除效率分别为1.52、1.79和1.56 日志。这些结果表明，S-nZVI和PMS对ARB和iARGs具有协同作用。再生分析表明ARB被有效地灭活。通过验证S-nZVI/PMS处理对结合转移的抑制作用，这项工作强调了一种有前途的抑制水平基因转移的替代技术。
Abstract
Antibiotic resistant bacteria (ARB) and the antibiotic resistance genes (ARGs) dissemination via plasmid-mediated conjugation have attracted considerable attentions. In this research, sulfidated nanoscale zerovalent iron (S-nZVI)/peroxymonosulfate (PMS) and S-nZVI/peroxydisulfate (PDS) process were investigated to inactivate ARB (Escherichia coli DH5α with RP4 plasmid, Pseudomonas. HLS-6 contains sul1 and intI1 on genome DNA sequence). S-nZVI/PMS system showed higher efficiency than S-nZVI/PDS on ARB inactivation. Thus, the optimal condition 28 mg/L S-nZVI coupled with 153.7 mg/L (0.5 mM) PMS was applied to remove both intracellular ARGs (iARGs) and ARB. The oxidative damage of ARB cell was systemically studied by cell viability, intracellular Mg2+ levels, the changes of extracellular and internal structure, integrity of cell walls and membranes and enzymatic activities. S-nZVI/PMS effectively inactivated ARB (~7.32 log) within 15 min. These effects were greatly higher than those achieved individually. Moreover, removal efficiencies of iARGs sul1, intI1 and tetA were 1.52, 1.79 and 1.56 log, respectively. These results revealed that S-nZVI and PMS have a synergistic effect against ARB and iARGs. The regrowth assays illustrated that the ARB were effectively inactivated. By verifying the inhibitory impacts of S-nZVI/PMS treatment on conjugation transfer, this work highlights a promising alternative technique for inhibiting the horizontal gene transfer.

https://www.sciencedirect.com/science/article/abs/pii/S0304389421018343