摘要
目标
副溶血性弧菌是一种国际食源性病原体，已导致许多人类感染病例。副溶血性弧菌菌株出现抗微生物耐药性（AMR）会阻碍抗菌药物的疗效。因此，本研究的目的是研究海鲜副溶血性弧菌Vp2015094的全基因组序列，并探索AMR的遗传机制。
方法
使用PacBio和Illumina平台进行全基因组测序。通过综合抗生素耐药性数据库中的耐药性基因识别器检测抗生素耐药性基因（ARGs）。将抗微生物质粒Vp2015094与来自不同来源的其他类似质粒进行比较。
后果
副溶血性弧菌Vp2015094含有两条染色体和两个质粒。在染色体和质粒pVp94-1上鉴定出ARGs。质粒pVp94-1携带四环素抗性基因（tetB、tetM、tetR、tetC）、氨基糖苷类抗性基因（aph（3′'）-Ib、aph（6）-Id）、磺酰胺抗性基因（sul2）、二氨基嘧啶抗性基因（dfrA6）、氟喹诺酮抗性基因（qnrVC6）、氯霉素抗性基因（floR）和对虾抗性基因（blaCARB-19），这些基因被转座酶基因包围。质粒pVp94-1是一个具有高转移频率的偶联质粒。
结论
副溶血性弧菌Vp2015094表现出由染色体和质粒介导的多重抗微生物耐药性。据推测，质粒和转座子负责ARGs的传播。这项研究提供了对海鲜副溶血性弧菌AMR遗传机制的了解，需要继续监测。
Abstract
Objectives
Vibrio parahaemolyticus is an international foodborne pathogen that has caused many human infection cases. The emergence of antimicrobial resistance (AMR) in V. parahaemolyticus strains can hinder the curative effect of antimicrobials. The aim of this study was therefore to investigate the whole genome sequence of V. parahaemolyticus Vp2015094 from seafood and to explore the genetic mechanisms of AMR.

Methods
The whole genome sequencing was performed using PacBio combined with Illumina platform. The antimicrobial resistance genes (ARGs) were detected by Resistance Gene Identifier in the Comprehensive Antibiotic Resistance Database. The antimicrobial-resistant plasmid of Vp2015094 was compared with other similar plasmids from different sources.

Results
Vibrio parahaemolyticus Vp2015094 contained two chromosomes and two plasmids. ARGs were identified on chromosome and plasmid pVp94-1. Plasmid pVp94-1 carried tetracycline resistance genes (tetB, tetM, tetR, tetC), aminoglycoside resistance genes (aph(3′')-Ib, aph(6)-Id), sulphonamide resistance genes (sul2), diaminopyrimidine resistance gene (dfrA6), fluoroquinolone resistance gene (qnrVC6), phenicol resistance gene (floR) and penam resistance gene (blaCARB-19), which were surrounded by transposase genes. Plasmid pVp94-1 was a conjugative plasmid with a high transfer frequency.

Conclusion
Vibrio parahaemolyticus Vp2015094 exhibited multi-antimicrobial resistance that was mediated by chromosome and plasmid. Plasmid and transposon were speculated to be responsible for the dissemination of ARGs. This study provided an understanding of the genetic mechanisms of AMR of V. parahaemolyticus from seafood, which needs continued monitoring.

https://www.sciencedirect.com/science/article/pii/S2213716522001278