摘要
      共享单车作为一种公共交通工具，为短途旅行提供了便利。同时它们也作为抗微生物耐药性（AR）细菌和抗微生物耐药性基因（ARGs）的潜在载体。然而，对从共享单车中收集的AR菌株和携带质粒的ARG的全基因组序列的了解仍然缺乏。在这里，我们使用HiSeq平台对从北京地铁站周围的共享自行车中分离的24株大肠杆菌进行了测序和分析。共享单车的分离株显示出14个ST和不同的基因型。两种产生blaNDM-5和blaCTX-M-199的ST167大肠杆菌具有16个抗性基因，4种质粒类型，与来自不同来源的ST167菌株相比，在核心基因组中显示出>95%的相似性。将通过Nanopore测序的携带blaNDM-5-或blaCTX-M-199的质粒与来源于人类和动物的带有blaNDM-5或blaCTX-M-199的载体进行比较。这两种ST167大肠杆菌在核心基因组和质粒图谱上与住院患者和农场动物的菌株具有高度相似性。我们的研究表明，ST167大肠杆菌保留在不同的环境中，并携带不同的质粒。对菌株如ST167的分析可以为预防或控制AR细菌在动物、人类和环境之间的传播提供有用的信息。
Abstract
Shared bikes as a public transport provide convenience for short-distance travel. Whilst they also act as a potential vector for antimicrobial resistant (AR) bacteria and antimicrobial resistance genes (ARGs). However, the understanding of the whole genome sequence of AR strains and ARGs-carrying plasmids collected from shared bikes is still lacking. Here, we used the HiSeq platform to sequence and analyze 24 Escherichia coli isolated from shared bikes around Metro Stations in Beijing. The isolates from shared bikes showed 14 STs and various genotypes. Two blaNDM-5 and blaCTX-M-199-producing ST167 E. coli have 16 resistance genes, four plasmid types and show >95% of similarities in core genomes compared with the ST167 E. coli strains from different origins. The blaNDM-5- or blaCTX-M-199-carrying plasmids sequencing by Nanopore were compared to plasmids with blaNDM-5- or blaCTX-M-199 originated from humans and animals. These two ST167 E. coli show high similarities in core genomes and the plasmid profiles with strains from hospital inpatients and farm animals. Our study indicated that ST167 E. coli is retained in diverse environments and carried with various plasmids. The analysis of strains such as ST167 can provide useful information for preventing or controlling the spread of AR bacteria between animals, humans and environments.

https://www.mdpi.com/2079-6382/11/8/1030