摘要
      越来越普遍的微囊藻水华和相关抗性基因的繁殖代表了全球环境问题。人工湿地是一种具有成本效益的污水处理技术。在本研究中，选择泽泻和三种工业副产品，即高炉矿渣、生物炭和锯末，建造小型CW装置。分析了它们修复毒性微囊藻的潜力及其对抗生素抗性基因（ARGs、sul1、sul2和intl1）行为的影响。木屑CW在短短2天内去除了约98.46%的微囊藻毒素细胞，其中检测到<0.37μg/L的残留微囊藻毒素（MC）-LR，去除效率>96.47%，这可能是由于基质上MC降解基因mlrA的相对丰度较高所致。木屑基CW中较低的目标ARG积累可能归因于较低的intl1相对丰度和微生物功能移动元素含量，这可能影响水平基因转移。在处理富营养化湖水的三个连续批次中，将六个木屑基CW单元组装成CW微宇宙。种植CW微宇宙对微囊藻和MC-LR的去除率分别在92.00%和95.88%之间以及86.48%和94.82%之间；显著高于未接种组（P＜0.05）。在种植的CWs中也观察到较少的目标ARGs积累。种植显著提高了氮去除率，这可能是由于通过宏基因组分析富集了基质中参与KEGG氮代谢途径的基因。
Abstract
Increasingly prevalent Microcystis blooms and the propagation of the associated resistance genes represent global environmental problems. Constructed wetlands (CWs) are a cost-effective technology used for wastewater treatment. In this study, the herb Alisma orientale and three industrial byproducts, namely, blast furnace slag, biochar, and sawdust, were selected to construct mini-CW units. Their potential to remediate toxic Microcystis and their influences on the behaviors of antibiotic-resistant genes (ARGs, sul1, sul2, and intl1) were analyzed. Approximately 98.46% of Microcystis cells were removed by the sawdust-based CW in just 2 d, wherein <0.37 μg/L residual microcystin (MC)-LR was detected, with a removal efficiency of >96.47%, which is potentially caused by the higher relative abundance of MC-degrading gene mlrA on the substrate. Lower target ARG accumulations in the sawdust-based CW may be attributed to the lower intl1 relative abundance and microbial function mobile element content, which could influence horizontal gene transfer. In three sequential batches for the treatment of eutrophic lake water, six sawdust-based CW units were assembled into CW microcosms. The efficiency of removal of Microcystis and MC-LR by planted CW microcosms ranged between 92.00% and 95.88% and between 86.48% and 94.82%, respectively; this was significantly (P < 0.05) higher than that by unplanted ones. Less accumulation of target ARGs was also observed in planted CWs. Planting considerably improved nitrogen removal, possibly owing to the enrichment of genes involved in the KEGG nitrogen metabolism pathway in the substrate through metagenomic analysis.

https://www.sciencedirect.com/science/article/abs/pii/S0045653521039552