摘要
      仔猪断奶前和断奶后阶段是肠道功能成熟的关键时期，抗生素耐药性细菌病原体的污染将威胁其肠道健康。噬菌体的存在也可以改变肠道中的细菌种群，但在新生仔猪肠道成熟过程中，抗生素抗性基因（ARG）的传播是否受到噬菌体的影响尚不清楚。因此，我们从3至28天收集的代表不同生长阶段的仔猪粪便样本中鉴定了肠道病毒组、ARGs和可移动遗传元件（MGE）。我们发现断奶仔猪的肠道病毒组波动很大，大多数与病毒相关的抗生素耐药性来自温和噬菌体，这表明新生猪肠中存在多药耐药性。我们的研究结果提供了对与肠道病毒组相关的ARG的全面了解，因此这代表了ARG水平转移到致病菌的潜在风险。
Abstract
The pre- and post-weaning stages for piglets are critical periods for the maturation of intestinal functions and contamination with antibiotic resistant bacterial pathogens will threaten their intestinal health. The presence of bacteriophage can also alter bacterial populations in the intestine but whether transmission of antibiotic resistance genes (ARG) is affected by phage during maturation of the neonatal piglet intestine is not known. We therefore identified the intestinal virome along with ARGs and mobile genetic elements (MGE) from piglet fecal samples collected from 3 to 28 days representing the different growth stages. We found wide fluctuations for the intestinal virome of weaning piglets and most virus - related antibiotic resistance was derived from temperate phage suggesting a reservoir of multidrug resistance was present in the neonatal porcine gut. Our results provide a comprehensive understanding of ARGs associated with the intestinal virome that therefore represents a potential risk for horizontal ARG transfer to pathogenic bacteria.

https://www.sciencedirect.com/science/article/abs/pii/S0378113522002449