摘要
出身背景
耐多药铜绿假单胞菌已成为对公众健康的危害，使医疗具有挑战性且无效。抗生素敏感性测试的全基因组测序为传统的微生物学方法提供了强有力的替代品。
客观的
本研究使用全基因组测序进行抗生素敏感性测试，评估了选定的铜绿假单胞菌临床菌株中抗生素耐药性基因的存在。
后果
对常见抗生素敏感的铜绿假单胞菌的全基因组测序显示存在4种抗生素耐药性基因类型，即fosA、catB7、blaPAO和blaOXA-50。耐药或耐多药铜绿假单胞菌的全基因组测序显示存在多种ARGs，如sul1、aac（3）-Ic、blaPAO、blaGES-1、blaGES-5 aph（3'）-XV、blaOXA-50、aacA4、catB7、aph（5'）-IIb、aadA6、fosA、tet（G）、cmlA1、aac（6'）Ib-cr和rmtF。
结论
抗生素耐药性基因的获得取决于假单胞菌对抗生素的耐药性。对抗生素抗性最高的菌株获得的抗生素抗性基因最多。多药耐药蛋白。铜绿假单胞菌产生对氨基糖苷类、β-内酰胺类、氟喹诺酮类、磺酰胺类、氯霉素和磷霉素类抗生素的耐药性基因。
Abstract
Background
Multidrug-resistant Pseudomonas aeruginosa has become a hazard to public health, making medical treatment challenging and ineffective. Whole-genome sequencing for antibiotic susceptibility testing offers a powerful replacement for conventional microbiological methods.

Objective
The present study evaluated the presence of antibiotic resistance genes in selected clinical strains of P. aeruginosa using whole-genome sequencing for antibiotic susceptibility testing.

Results
Whole-genome sequencing of P. aeruginosa susceptible to common antibiotics showed the presence of 4 antibiotic resistance gene types, fosA, catB7, blaPAO, and blaOXA-50. Whole genome sequencing of resistant or multidrug-resistant P. aeruginosa showed the presence of multiple ARGs, such as sul1, aac(3)-Ic, blaPAO, blaGES-1, blaGES-5 aph (3’)-XV, blaOXA-50, aacA4, catB7, aph(3’)-IIb, aadA6, fosA, tet(G), cmlA1, aac(6’)Ib-cr, and rmtF.

Conclusion
The acquisition of antibiotic resistance genes was found to depend on the resistance of Pseudomonas to antibiotics. The strain with the highest resistance to antibiotics had the highest acquisition of antibiotic resistance genes. MDR-P. aeruginosa produces antibiotic resistance genes against aminoglycoside, β-lactam, fluoroquinolones, sulfonamides, phenicol, and fosfomycin antibiotics.

https://www.tandfonline.com/doi/full/10.2147/IDR.S389959