摘要
      有机施肥是抗生素抗性基因在农业生态系统中传播的主要原因，这大大增加了抗生素抗性基因获得及其传播到人类食物链中的风险。蚯蚓是地下和地上联系中最重要的土壤动物之一，硅有利于土壤健康和植物抗逆性。本研究旨在通过高通量qPCR探讨不同改良策略（蚯蚓和/或硅）及其相关影响因素对ARGs缓解的影响。结果表明，蚯蚓和硅肥的施用降低了根际土壤中ARGs的绝对丰度，无论是单独施用还是联合施用。根据结构方程模型和随机森林分析，流动遗传元素是促进ARGs转移的主要因素，处理以直接或间接的方式影响ARGs。我们的研究结果强调了“根际效应”在缓解抗生素耐药性方面的作用，并表明硅肥和蚯蚓可以被视为一种可持续的天然解决方案，以缓解高风险ARGs在土壤-植物系统中的传播。我们的研究结果为制定阻止ARGs在农业生态系统中传播的策略提供了指导。
Abstract
Organic fertilization is a major contributor to the spread of antibiotic resistance genes (ARGs) in the agroecosystem, which substantially increases the risk of ARGs acquisition and their transmission into human food chains. Earthworms are among the most vital soil faunas involved in the link between belowground and aboveground, and silicon is beneficial for soil health and plant stress resistance. This study aims to explore the effect of different amendment strategies (earthworm and/or silicon) and the related influencing factors on the alleviation of ARGs using high-throughput qPCR. The results showed that the application of earthworms and silicon fertilizers reduced the absolute abundance of ARGs in the rhizosphere soils, either singly or in combination. According to the structural equation model and random forest analysis, mobile genetic elements are the major factors enhancing ARGs transfers and the treatment affects ARGs in direct or indirect ways. Our results highlight the role of “rhizosphere effect” in alleviating antibiotic resistance and suggest that silicon fertilizers, together with the earthworms, can be considered as a sustainable and natural solution to mitigate high-risk ARGs spread in the soil-plant systems. Our findings provide guidance in formulating strategies for halting the spread of ARGs in the agroecosystem.

https://www.sciencedirect.com/science/article/abs/pii/S0269749122021157