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解读中国东部和西部不同海拔城市污水处理厂的抗生素耐药性和微生物群落

发布者:抗性基因网 时间:2023-06-08 浏览量:219

摘要
      抗生素耐药性基因作为新出现的环境污染物,对公众健康和生态系统构成了严重的全球风险。城市污水处理厂(WWTP)是通过移动遗传元件(MGE)将ARGs传播和繁殖到接收水体中的关键发射器。然而,在两个不同的海拔WWTP中,对ARGs的多样性、丰度和潜在宿主的全面深入解读是罕见的。在这项工作中,我们揭示了来自两个不同地理区域的六个污水处理厂的抗性基因和微生物群落的海拔分布特征:低海拔(LE)区域(山东,海拔10-22米)和高海拔(HE)区域(甘肃,海拔1520-1708米)。在抗性基因的多样性和相对丰度方面观察到显著的海拔变化。废水处理可以显著降低ARGs和MGE的浓度,分别降低约1-2和2-3个数量级。然而,69.95%以上的抗性基因在废水中富集。特别是,24个ARG亚型、3个MGE亚型和59个细菌属在所有样本中都是持久的。在LE区发现了更多ARGs的潜在宿主,在HE区发现了更丰富的人类肠道微生物群。这项工作为控制ARG的传播及其管理和评估提供了有用的信息,从而降低了污水处理厂中ARG的风险。
Abstract
Antibiotic resistance genes (ARGs) as emerging environmental contaminants pose severe global risks to public health and ecosystems. Municipal wastewater treatment plants (WWTPs) are crucial transmitters for the dissemination and propagation of ARGs into receiving water bodies via mobile genetic elements (MGEs). However, the comprehensive and deep deciphering of the diversity, abundance, and potential hosts of ARGs in two distinct altitudinal WWTPs is scarce. In this work, we revealed the elevational distribution characteristics of the resistance genes and microbial community of six WWTPs from two distinct geographical zones: a low−elevation (LE) region (Shandong, 10–22 m above sea level) and a high−elevation (HE) region (Gansu, 1,520–1,708 m above sea level). Significant elevational variations in the diversity and relative abundance of resistance genes were observed. Wastewater treatment could significantly reduce the concentrations of ARGs and MGEs by about 1–2 and 2–3 orders of magnitude, respectively. However, above 69.95% of resistance genes were enriched in effluent. In particular, 24 ARG subtype, 3 MGE subtypes, and 59 bacterial genera were persistent in all samples. More potential hosts for ARGs in LE region and more abundant human gut microbiota in HE region were identified. This work provides helpful information for controlling the spread of ARGs for their management and assessment, thereby mitigating the risks of ARGs in WWTPs.

https://www.sciencedirect.com/science/article/abs/pii/S0043135422014063