摘要
      堆肥中增强型抗生素抗性基因（ARGs）的减少对于降低农业生产中ARGs传播的风险很重要。氢car作为一种具有吸附和催化性能的功能性碳材料，在许多应用中都有应用。本研究调查了添加水炭对鸡粪堆肥中细菌群落、可移动遗传元素（MGE）和ARGs的影响。添加2%、5%和10%的水炭（干重）可使堆肥产品中的目标ARG和MGE总数分别减少40.13–55.33%和23.63–37.23%。氢钙改变了堆肥过程中细菌种群的演替，降低了潜在病原体的丰度，促进了微生物在氨基酸和碳水化合物代谢中的活性。ARGs的一个重要的可能微生物宿主是厚壁菌门。如偏最小二乘路径建模分析所示，水顾问通过改变间接影响ARG分布的环境因素，直接影响宿主微生物和MGE。总之，在堆肥中添加水炭是一种简单有效的促进ARGs去除的方法。
Abstract
The reduction of enhanced antibiotic resistance genes (ARGs) in compost is important to mitigate the risk of ARG transmission in agricultural production. Hydrochar is used in many applications as a functional carbon material with adsorption and catalytic properties. This study investigated the effects of hydrochar addition on bacterial communities, mobile genetic elements (MGEs), and ARGs in chicken manure composting. The addition of 2%, 5%, and 10% hydrochar (dry weight) reduced the total numbers of target ARGs and MGEs in the compost products by 40.13–55.33% and 23.63–37.23%, respectively. Hydrochar changed the succession of the bacterial population during composting, lowering the abundance of potential pathogens and promoting microbial activity in amino acid and carbohydrate metabolism. A significant possible microbial host for ARGs was found to be Firmicutes. Hydrochar was found to affect the host microorganisms and MGEs directly by altering environmental factors that indirectly impacted the ARG profiles, as shown by partial least squares pathway modeling analysis. In conclusion, the addition of hydrochar to compost is a simple and effective method to promote the removal of ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0304389423007422