摘要
      畜牧场的粪便是抗生素抗性基因（ARGs）的蓄水池，这些基因可以传播到周围的土壤和空气中，给人类健康带来风险。在这项研究中，探讨了ARGs在畜牧场的季节性传播及其对人类暴露的影响。实验结果表明，ARGs的丰度基本上为粪便>土壤>空气，并具有显著的季节依赖性。猪粪便中的总ARGs在秋季（109.7拷贝g−1）和冬季（1010.0拷贝g−2）相对较高，而在夏季（105.0拷贝g−3）较低。同样，土壤和空气中的ARG总量在夏季也最低。ARGs、整合素intI1和细菌群落之间存在相关性。总有机碳是影响ARGs在粪便中分布的重要因素，pH和水分含量对土壤ARGs有显著影响。空气中整合素intI1和ARGs的每日摄入量分别为10°.5拷贝h−1和102.3拷贝h−1。假单胞菌是粪便中blaTEM-1的潜在致病宿主，假单胞菌和不动杆菌是土壤中多种ARGs的潜在致病寄主，而空气中的ARGs不会迁移到病原体中。
Abstract
Feces in livestock farms is a reservoir of antibiotic resistance genes (ARGs), which can disseminate into surrounding soil and air, bringing risks to human health. In this study, seasonal dissemination of ARGs in a livestock farm and implications for human exposure was explored. The experimental results showed that ARGs abundance basically ranked as feces > soil > air, and significant seasonal dependence was observed. The total ARGs in pig feces was relatively higher in autumn (109.7 copies g−1) and winter (1010.0 copies g−1), and lower in summer (105.0 copies g−1). Similarly, the lowest total ARGs in soil and air were also observed in summer. There were correlations among ARGs, integron intI1, and bacterial community. Total organic carbon was an important factor affecting ARGs distribution in the feces, and pH and moisture content significantly affected soil ARGs. The daily intakes of integron intI1 and ARGs from air were 10°.5 copies h−1 and 102.3 copies h−1 for human exposure, respectively. Pseudomonas was a potential pathogenic host of blaTEM-1 in feces, Pseudomonas and Acinetobacter were potential pathogenic hosts of multiple ARGs in soil, while ARGs in air did not migrate into pathogens.

https://www.sciencedirect.com/science/article/abs/pii/S0301479722022113