摘要
      有效减少异位发酵系统（EFS）中的抗生素抗性基因（ARGs）对于实际生产至关重要。在本研究中，进行了三个实验来探索如何有效地去除EFS中的ARGs。结果表明，在稻壳木屑填料中，ARGs很容易富集；同时添加漆酶和纤维素酶抑制ARGs，主要是通过增加可溶性碳水化合物浓度和促进腐殖酸浓度；在稻壳木屑中添加玉米秸秆可以改善碳源结构，提高纤维素酶活性，从而降低ARGs的丰度。总之，本研究为减少EFS中ARGs的威胁提供了指导，为安全利用粪肥资源铺平了潜在的道路。
Abstract
Effectively reduce antibiotic resistance genes (ARGs) in ectopic fermentation system (EFS) is essential for practical production. In this study, three experiments were performed to explore how to remove ARGs in EFS effectively. Results demonstrated that ARGs were easily enriched in rice-husk-sawdust padding; simultaneous addition of laccase and cellulase suppressed the ARGs, mainly by increasing soluble carbohydrate concentration and promoting humic acid concentration; addition of corn stalks into rice-husk-sawdust decreased the abundance of ARGs by improving the carbon source structure and enhancing cellulase activity. In conclusion, the present study provides a guidance to reduce the threat of ARGs in EFS, which paved a potential pathway to safely utilize manure resources.

https://www.sciencedirect.com/science/article/abs/pii/S096085242300490X