摘要
      了解航天器装配洁净室中的微生物群落和抗生素耐药性基因（ARGs）对航天器微生物控制和宇航员安全至关重要。然而，关于ARG谱及其与此类环境中微生物群的关系的报道很少。在本研究中，我们评估了典型航天器装配洁净室空气灰尘和表面环境中的细菌群落和ARGs。我们的研究结果表明，表面和空气尘埃之间的细菌组成存在显著差异，因为它们属于两种不同的生态状态。芽孢杆菌和不动杆菌在空气样本中显著富集。细菌群落网络分析揭示了空气样本中较低的拓扑参数和细菌网络的稳健性。我们还观察到一些典型的ARG在地表和空气尘埃样本之间的不同分布模式。值得注意的是，与在空气中相比，ermB基因表现出相对较高的拷贝数，并且在表面环境中富集。总的来说，我们的研究深入了解了复杂的微生物群落以及ARGs在航天器组件洁净室中的分布和转移，并为制定针对ARGs的控制策略提供了重要的投入。
Abstract
Understanding the microbial communities and antibiotic resistance genes (ARGs) in spacecraft assembly cleanrooms is crucial for spacecraft microbial control and astronaut safety. However, there have been few reports of ARG profiles and their relationship with microbiomes in such environments. In the present study, we assessed the bacterial community and ARGs in the air dust and surface environments of a typical spacecraft assembly cleanroom. Our results show a significant difference in bacterial composition between surfaces and air dust, as they belong to two distinct ecostates. Bacillus and Acinetobacter were significantly enriched in the air samples. Bacterial community network analysis revealed lower topological parameters and robustness of bacterial networks in the air samples. We also observed different distribution patterns of some typical ARGs between surface and air dust samples. Notably, the ermB gene exhibited a relatively high copy number and was enriched in the surface environment, compared to that in the air. Overall, our study provides insight into the complex microbial community and the distribution and transfer of ARGs in spacecraft assembly cleanrooms, and offers important input for developing control strategies against ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0269749123006152