摘要
      空气中PM2.5对健康的影响机制是复杂的，肠道菌群可能在其中发挥作用。本研究旨在研究吸入PM2.5的大鼠肠道菌群的变化，进一步分析大鼠肠道微生物群中抗生素耐药性基因的表达。收集中国福建省空气中的PM2.5，建立PM2.5吸入大鼠模型。采用宏基因组方法对粪便样本进行测序。肠道菌群种类注释，常见功能对吸入PM2.5的大鼠和正常对照大鼠的数据库注释和ARGs进行了分析。结果表明，在门水平上，吸入PM2.5大鼠的厚壁菌门数量显著减少，而拟杆菌门数量增加。此外，从属水平到种水平，PM2.5吸入组的肠道菌群组成与对照组完全不同。特别是PM2.5暴露会改变ARGs的表达在肠道菌群中。在吸入PM2.5的Wistar大鼠中，rpoB2、Tet44、TetW和TetO的ARGs显著增加。控制空气污染和减少PM2.5排放对维持公众健康至关重要。
Abstract
The mechanism of PM2.5 in the air on health is complicated, in which the gut flora may play a role.The work aimed to study the changes of intestinal microflora in PM2.5 inhaled rats, further, to analyze the expression of antibiotic resistance genes (ARGs) in intestinal flora of the rats. The PM2.5 in the air of Fujian Province of China was collected to establish the rat model of PM2.5 inhaled. The fecal samples were sequenced by metagenomic method. Intestinal flora species annotation, common function
database annotation and ARGs were analyzed between PM2.5 inhaled rats and normal control rats.The results showed that the number of Firmicutes was significantly decreased while that of Bacteroides was increased in the PM2.5 inhaled rats compared with the control rats at phylum level. In addition, from genus level to species level, the composition of intestinal flora in PM2.5 inhaled group was totally different from those of control group. Especially, PM2.5 exposure can change the expression of ARGs
in intestinal flora. The ARGs of rpoB2, Tet44, TetW and TetO increased significantly in the PM2.5 inhaled Wistar rats.Controlling air pollution and reducing PM2.5 emissions are crucial to maintain public health.

http://www.pjoes.com/pdf-158760-87580?filename=Can%20PM2.5%20Exposure%20Change.pdf