摘要
      抗生素耐药性细菌（ARB）和抗生素耐药性基因（ARGs）已被认为是新出现的污染物。通过在水生环境中失活ARB并通过氧化处理过程降解其ARGs，可以限制抗生素耐药性的传播，但迄今为止，尚未报道产生基因降解中间体的情况。在本研究中，一种新型的热解后生物杂交碳(Co@PS-BC)制备用于过氧一硫酸盐（PMS）活化以消除大肠杆菌DH5α基ARB及其ARGs（blaTEM-1和NPTII）。重要的是，通过纳米孔测序和Illumina测序分析了ARGs的基因降解中间体。生物信息学分析结果表明，基因降解中间体具有丰富的GGC基序、相对较高的GC含量和断点对鸟嘌呤的碱基偏好。总的来说，我们的工作开发了一种从水中有效消除ARB和ARGs的新方法，并深入了解了基因降解中间体的组成和碱基偏好。
Abstract
Antibiotic resistance bacteria (ARB) and antibiotic resistance genes (ARGs) have been recognized as emerging pollutants. It is accessible to restrict disseminating antibiotic resistance by inactivating ARB and degrading its ARGs by oxidation treatment processes in aquatic environment, but until now the producing gene-degrading intermediates are not reported. In this study, a novel post-pyrolysis bio-hybridcarbon (Co@PS-BC) was prepared for peroxymonosulfate (PMS) activation to eliminate both E. coli DH5α-based ARB and its ARGs (blaTEM-1 and NPTII). Importantly, gene-degradation intermediates of ARGs were analyzed by Nanopore sequencing and Illumina sequencing. The results of bioinformatic analyses showed that the gene-degrading intermediates held the abundant GGC motifs, relatively high GC content and base preference of breakpoints toward guanine. Overall, our work develops a novel approach for effectively eliminating ARB and ARGs from water, and provides an insight into the composition and base preference of gene-degrading intermediates.

https://www.sciencedirect.com/science/article/abs/pii/S1385894723005405