摘要
      依靠水流的高流动性，抗生素抗性基因（ARGs）在水中的传播往往会加剧和扩大。它对更大范围的环境造成了负面影响。ARGs的传播监测和有效降低其在水中浓度的方法成为人们关注的焦点。具有抗菌作用的绿色化学物质，如茶多酚（TP）和儿茶素（CA），已被认为是去除水环境中ARGs的辅助消毒剂。然而，TP和CA在体外抗生素胁迫下的抗菌性能仍然缺乏足够的研究。结果表明，在四环素的压力下，在含有TP和CA的水样中可以观察到比不含有成分的水样更多的操作分类单元。在低浓度TP或CA（1 mg/L）下，随着ARGs浓度和微生物群落多样性的增加，出现了意想不到的增加。此外，在四环素胁迫下，对TPs的抑制作用随着inti1和tetC的增加而增强，但随着tetA的增加则减弱。而CA在四环素压力下显著降低了tetC和tetA的丰度。这项研究表明，在水系统中抗生素的压力下，TP和CA能够减缓ARGs的发展。
Abstract
Relying on the high mobility of water flow, the dissemination of antibiotic resistance genes (ARGs) in the water tends to be exacerbated and enlarged. It caused negative impacts on a wider scope of the environment. The ARGs dissemination monitoring and the methods efficiently reducing their concentration in water became the focus of interest. Green chemicals with antibacterial effects such as tea polyphenols (TPs) and catechins (CA) have been considered as auxiliary disinfectants for ARGs removal in the water environment. However, the antibacterial performance of TPs and CA under the stress of external antibiotics still lacks sufficient research. The results show that more operational taxonomic units can be observed in water samples with TPs and CA than in those without the ingredients under pressure of tetracycline. An unexpected increase along with the increase of ARGs concentrations and the diversity of microbial communities under the low-concentration TPs or CA (1 mg/L). Besides, under the stress of tetracycline, the inhibition of TPs was detected to be strengthened for increase of inti1 and tetC but weakened towards for the increase of tetA. Whilst CA substantially diminished abundances of tetC and tetA under tetracycline pressure. This research demonstrated that TPs and CA are able to assuage development of ARGs under the pressure of antibiotic in water system.

https://www.sciencedirect.com/science/article/pii/S0147651323001793