摘要
      在本研究中，我们建议以连续流操作模式操作Fe0催化的微藻基MFC，以探索其抗生素去除和相关的生物机制，以应对MFC中阳极酸化、阴极电解质的二次污染、抗生素抗性基因（ARGs）的积累和氮磷去除效率低的问题。结果表明，与间歇运行的MFC相比，藻类阴极MFC对COD、NH3-N和TN的去除率分别提高了14.24%、20.27%和32.27%。Fe0的添加不仅显著提高了金霉素的去除率（P<0.05），而且显著降低了ARGs和可移动遗传元件（MGE）的传播风险。Fe0的加入在阳极中形成厌氧氨氧化和Feammox耦合反硝化机制，以实现强化反硝化。在Fe0=20 mg/L时，AC-MFC的最高输出电压为420 mV，对应的功率密度为13.39 W.m−3，是未注入Fe0时的两倍。优势门主要包括厚壁菌门，第二优势门包括拟杆菌门和变形菌门。在添加Fe0。本研究表明，在连续流操作模式下，Fe0催化的微藻MFC不仅显著提高了抗生素的去除率，促进了氮磷的去除，而且有效地控制了ARGs的转移。研究结果可为抗生素废水处理和ARGs的控制提供参考。
Abstract
In this study, we propose to operate Fe0-catalyzed microalgae-based MFCs in continuous flow operation mode to explore their antibiotic removal and related biological mechanisms in response to the problems of anode acidification, secondary contamination of cathode electrolyte, and accumulation of antibiotic resistance genes (ARGs) and low efficiency of nitrogen and phosphorus removal in MFCs. The results showed that the removal rates of COD, NH3-N and TN were improved by 14.24 %, 20.27 % and 32.27 %, respectively, in the algae cathode MFC (AC-MFC) compared with the intermittently operated MFCs. The addition of Fe0 not only significantly improved the removal of Chlortetracycline (P < 0.05), but also significantly reduced the risk of transmission of ARGs and mobile genetic elements (MGEs). The addition of Fe0 formed an anaerobic ammonia oxidation and Feammox coupled denitrification mechanism in the anode to achieve enhanced denitrification. At Fe0 = 20 mg/L, the highest output voltage of AC-MFC was 420 mV, corresponding to a power density of 13.39 W.m−3, which was twice as high as when Fe0 was not injected. The dominant phylum mainly included Firmicutes, and the second dominant phylum included Bacteroidota and Proteobacteria. Anaeroarcus spp. with Fe reduction function became the dominant genus in MFC after the addition of Fe0. This study demonstrated that the Fe0-catalyzed microalgal MFCs in continuous flow operation mode not only significantly enhanced antibiotic removal and promoted nitrogen and phosphorus removal, but also effectively controlled the transfer of ARGs. The results can provide a reference for antibiotic wastewater treatment and the control of ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S2214714423002209