摘要
      本研究研究了过乙酸（PAA）/紫外线-C发光二极管（UVC-LED）处理对抗生素抗性基因（ARG）失活和降解的影响。使用转化测定法测量质粒pUC19中编码的氨苄青霉素抗性（ampR）基因的失活速率常数。PAA（10–50 ppm）/UVC-LED处理的pUC19的失活速率常数随着PAA浓度的增加而增加。在50ppm的PAA下，失活速率常数达到其最大值：0.16cm2/mJ。通过定量聚合酶链式反应（qPCR）获得了四个ampR扩增子（192400603和851bp）的降解速率常数。UVC-LED处理时pUC19质粒的平均降解速率常数为1.45×10−1 cm2/mJ，PAA/UVC-LED治疗时为1.17 cm2/mJ。使用PAA/UVC-LED，ampR基因在番茄和卷心菜表面的失活率分别为2.69×10−2 cm2/mJ和2.48×10−2m2/mJ。通过对每种新鲜农产品的颜色质量评估，证实使用PAA/UVC-LED处理可以有效控制ARG，而不会影响新鲜农产品颜色质量。在这项研究中，使用PAA/UVC-LED处理的ARG控制效果在水中和新鲜农产品表面上都得到了确认，这些表面有很高的ARG暴露风险。
Abstract
This study investigates the effects of peracetic acid (PAA)/ultraviolet-C light-emitting-diode (UVC-LED) treatment on the deactivation and degradation of the antibiotic-resistance gene (ARG). The deactivation rate constant for the ampicillin-resistance (ampR) gene encoded in plasmid pUC19 was measured using the transformation assay. The deactivation rate constant of pUC19 treated by PAA (10–50 ppm)/UVC-LED grew with an increasing concentration of PAA. At a PAA of 50 ppm, the deactivation rate constant reached its maximum: 0.16 cm2/mJ. Degradation rate constants for four ampR amplicons (192, 400, 603, and 851 bp) were obtained via the quantitative polymerase chain reaction (qPCR). The average degradation rate constant for the pUC19 plasmid was 1.45 × 10−1 cm2/mJ with UVC-LED treatment, and 1.17 cm2/mJ with the PAA/UVC-LED treatment. Using PAA/UVC-LED, the deactivation rates for the ampR gene on the tomato and cabbage surfaces were 2.69 × 10−2 cm2/mJ and 2.48 × 10−2 cm2/mJ, respectively. Through color quality evaluation for each fresh produce confirmed that ARG could be effectively controlled using the PAA/UVC-LED treatment without affecting the color quality of fresh produce. In this study, the ARG control effect using the PAA/UVC-LED treatment was identified both in water and on fresh produce surfaces, which have a high risk of ARG exposure.

https://www.sciencedirect.com/science/article/pii/S0956713522006715