摘要
      耐多药铜绿假单胞菌有可能引起医院感染。在这项研究中，对从孟加拉国分离的两种极具耐药性的新菌株SRS1和SRS4进行了全基因组测序。SRS1的基因组草案大小为6.8Mbp，SRS4的基因组草案为7.0Mbp。计算机分析预测SRS1的基因组有82个，SRS4的基因组有75个抗生素抗性基因（ARGs）。抗体谱结果显示，SRS1和SRS4都对β-内酰胺类、喹诺酮类和氨基糖苷类抗生素家族的多个成员具有耐药性。此外，SRS1和SRS4的基因组被预测具有多种可移动元件，如原噬菌体和质粒。与野生型PAO1和另一种耐药铜绿假单胞菌菌株JNQH-PA57的比较基因组分析显示，SRS1和SRS4比PAO1或JNQH-PA57含有更多的抗生素抗性基因，如AAC（6´）-II、ANT（2´´）-Ia、ANT。这项研究表明了基因组研究的重要性，以了解ARGs在孟加拉国铜绿假单胞菌菌株中的分布，从而证明导致多药耐药性的机制。
Abstract
Multidrug-resistant P. aeruginosa has potential to cause nosocomial infections. In this study, whole-genome sequencing was performed of two extremely drug-resistant novel strains SRS1 and SRS4 isolated from Bangladesh. The size of draft genome of SRS1 is 6.8 Mbp, and 7.0 Mbp for SRS4. In silico analysis predicted that the genome of SRS1 has 82 and SRS4 has 75 antibiotic-resistant genes (ARGs). Antibiogram results revealed that both SRS1 and SRS4 were resistant to multiple members of the antibiotic groups of β−lactam, quinolones, and aminoglycosides families. In addition, the genomes of both SRS1 and SRS4 were predicted to have multiple mobile elements like prophages and plasmids. Comparative genome analysis with wildtype PAO1 and another drug-resistant P. aeruginosa strain JNQH-PA57 revealed that SRS1 and SRS4 contain more antibiotic resistance genes like AAC (6´)-II, ANT (2´´)-Ia, ANT (3´´)-IIa, OXA-395, PME-1, qacEΔ1, tet(A), tet(D), VEB-9 than PAO1 and JNQH-PA57. This study shows the importance of the genomic study to understand the distribution of ARGs in Bangladeshi P. aeruginosa strains to demonstrate the mechanisms responsible for multi drug resistance.

https://www.banglajol.info/index.php/BRC/article/view/63601