摘要
      抗菌药物已被用于改善动物福利、食品安全和食品安全，促进抗微生物（AMR）细菌的出现、选择和传播。在这项研究中，从达卡市的冷冻鸡肉样本中分离出50株大肠杆菌。通过圆盘扩散法评估抗生素敏感性模式，并最终使用聚合酶链式反应（PCR）筛选抗微生物耐药性基因（ARG）的存在。在160个样本中，在50个样本中观察到大肠杆菌的流行率（31.25%）。所有这些分离株都对至少一种抗菌剂具有耐药性，52.0%的分离株对4-7种不同的抗菌剂具有抗性。对四环素（66.0%）表现出高耐药性，其次是对红霉素（42.0%）、氨苄青霉素和链霉素（38.0%）以及磺酰胺（28.0%）的耐药性。此外，最常见的ARGs是tet（A）（6.60%）、ereA（64.0%）、tet（B）（60.0%）、aadA1和sulI（56.0%）、blaCITM（48.0%）和blaSHV（40.0%）。约90.0%的分离株具有多药耐药性。这项研究首次揭示了肉鸡大肠杆菌AMR的现状，这有助于临床控制疾病，并有助于制定政策和指南，以减少孟加拉国肉鸡生产中的AMR。
Abstract
Antimicrobials have been used to improve animal welfare, food security, and food safety that promote the emergence, selection, and dissemination of antimicrobial-resistant (AMR) bacteria. In this study, 50 E. coli were isolated from frozen chicken meat samples in Dhaka city. Antibiotic sensitivity patterns were assessed through the disk diffusion method and finally screened for the presence of antimicrobial resistance genes (ARG) using the polymerase chain reaction (PCR). Among the 160 samples, the prevalence of E. coli was observed in fifty samples (31.25%). All of these isolates were found resistant to at least one antimicrobial agent, and 52.0% of the isolates were resistant against 4–7 different antimicrobials. High resistance was shown to tetracycline (66.0%), followed by resistance to erythromycin (42.0%), ampicillin and streptomycin (38.0%), and sulfonamide (28.0%). In addition, the most prevalent ARGs were tet(A) (66.0%), ereA (64.0%), tet(B) (60.0%), aadA1 and sulI (56.0%), blaCITM (48.0%) and blaSHV (40.0%). About 90.0% of isolates were multidrug resistant. This study reveals for the first time the current situation of E. coli AMR in broilers, which is helpful for the clinical control of disease as well as for the development of policies and guidelines to reduce AMR in broilers production in Bangladesh.

https://www.mdpi.com/2079-6382/12/1/41