摘要

进行了乳酸菌（LAB）在鱼类消化道中的细菌素筛选和表征。从6种鱼类的胃肠道中筛选出四百个LAB分离物; （鲈鱼），Channa striata（条纹蛇头鱼），Clarias batrachus（鲶鱼），Orecochromis niloticus（尼罗罗非鱼），Barbonymus gonionotus（爪哇鱼）和Pangasianodon hypophthalmus（条纹鲶鱼）。一种来自鲈鱼的有效分离物能够产生细菌素并命名为Sb2。对17种细菌目标菌株进行无细胞上清液（CFS，pH 6.0）的抗菌活性测试。 Sb2的CFS显示对腐败菌和致病菌的生长抑制。最大的细菌素活性为12,800 Au / ml，对抗Lb. sakei subsp。清酒JCM 1157T在30℃的最佳培养条件下获得18小时。其胰蛋白酶，α-胰凝乳蛋白酶和蛋白酶K的蛋白水解酶部分抑制其细菌素活性，分别为50％，75％和96.87％。在高达100°C的高温下稳定30分钟。然而，当它在100℃温育60分钟和4℃温育7天时，其活性降低至50％（从12,800至6,400 Au / ml）。因此，由Sb2产生的细菌素可以用作生物防腐剂。

Screening and characterization of bacteriocin from lactic acid bacteria (LAB) in fish gastrointestinal tract were conducted. Four hundred isolates of LAB were screened from gastrointestinal tract of 6 fish species; Lates calcarifer (seabass), Channa striata (striped snake-head fish), Clarias batrachus (catfish), Orecochromis niloticus (nile tilapia), Barbonymus gonionotus (javanese barb) and Pangasianodon hypophthalmus (striped catfish). One effective isolate from seabass was able to produce bacteriocin and designated Sb2. Antibacterial activity test of cell free supernatant (CFS, pH 6.0) was performed against 17 bacteria target strains. CFS of Sb2 displayed growth inhibition of both spoilage and pathogenic bacteria. The maximum bacteriocin activity of 12,800 Au/ml against Lb. sakei subsp. sakei JCM 1157T was obtained at the optimum cultivation condition of 30°C for 18 hr. Its bacteriocin activity was partially inhibited by proteolytic enzyme of trypsin, a-chymotrypsin and proteinase K for 50%, 75% and 96.87%, respectively. It was stable at high temperature up to 100°C for 30 min. However, its activity was reduced to 50% (from 12,800 to 6,400 Au/ml) when it was incubated at 100°C for 60 min and 4°C for 7 days. As a result of this, bacteriocin produced from Sb2 could be used as biopreservative.

https://www.tci-thaijo.org/index.php/APST/article/view/84069