摘要 

评估了猪粪堆肥（SMC），庭院垃圾堆肥（YWC）或食物垃圾堆肥（FWC）对土壤中抗生素抗性基因丰度的影响。在田间试验中商业规模施用堆肥之后，定期采样土壤十年，并将其存档风干。提取土壤DNA并通过qPCR定量基因目标。与未处理的对照土壤相比，所有3种修正类型增加了4年后基因目标的丰度。在用SMC修正的土壤中，几个基因靶标的丰度比在接受YWC或FWC的土壤中高得多。在SMC治疗中，基因靶ermB在十年后仍保持较高水平。在应用后的十年中，梭菌在SMC修正的土壤中显着更丰富。 8％的梭菌属。来自SMC治疗的分离物携带ermB。总体而言，在土壤中添加有机修饰物有可能增加抗生素抗性基因的丰度。粪便来源的修饰，如SMC，还会携带携带抗生素抗性基因的细菌。环境顽拗性梭菌和它们携带的抗生素抗性基因在使用SMC后的现场条件下将持续多年。

The impact of amendment with swine manure compost (SMC), yard waste compost (YWC), or food waste compost (FWC) on the abundance of antibiotic resistance genes in soil was evaluated. Following a commercial-scale application of the composts in a field experiment, soils were sampled periodically for a decade, and archived air-dried. Soil DNA was extracted and gene targets quantified by qPCR. Compared with untreated control soil, all 3 amendment types increased the abundance of gene targets for up to 4 years postapplication. The abundance of several gene targets was much higher in soil amended with SMC than in soil receiving either YWC or FWC. The gene target ermB remained higher in the SMC treatment for a decade postapplication. Clostridia were significantly more abundant in the SMC-amended soil throughout the decade following application. Eight percent of Clostridium spp. isolates from the SMC treatment carried ermB. Overall, addition of organic amendments to soils has the potential to increase the abundance of antibiotic resistance genes. Amendments of fecal origin, such as SMC, will in addition entrain bacteria carrying antibiotic resistance genes. Environmentally recalcitrant clostridia, and the antibiotic resistance genes that they carry, will persist for many years under field conditions following the application of SMC.

http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2017-0642