摘要

过度使用抗生素已经在水产养殖系统中引起抗生素抗性基因（ARG）的繁殖。本研究首先探讨了典型海水养殖场ARGs概况，包括使用宏基因组学方法的传统和循环系统。确定了属于21个ARGs类型的50个ARGs亚型，显示了沿海工业海水养殖系统中ARGs的广谱谱。 ARGs具有多种抗生素耐药性已经出现在尿液系统中。 ARG和微生物分类群之间的共现模式表明，Proteobacteria和Bacteroidetes是工业海水养殖系统中潜在的ARGs主要宿主。在海水养殖系统中的典型硝化细菌如Nitrospinae也带有一些抗性基因。鱼塘和污水处理装置中ARG的相对丰度相对较高。调查表明，工业海水养殖系统是沿海地区重要的ARGs水库，表明再循环系统在ARG污染控制方面的关键作用。

The overuse of antibiotics has posed a propagation of antibiotic resistance genes (ARGs) in aquaculture systems. This study firstly explored the ARGs profiles of the typical mariculture farms including conventional and recirculating systems using metagenomics approach. Fifty ARGs subtypes belonging to 21 ARGs types were identified, showing the wide-spectrum profiles of ARGs in the coastal industrial mariculture systems. ARGs with multiple antibiotics resistance have emerged in the mariculure systems. The co-occurrence pattern between ARGs and microbial taxa showed that Proteobacteria and Bacteroidetes were potential dominant hosts of ARGs in the industrial mariculture systems. Typical nitrifying bacteria such as Nitrospinae in mariculture systems also carried with some resistance genes. Relative abundance of ARGs in fish ponds and wastewater treatment units was relatively high. The investigation showed that industrial mariculture systems were important ARGs reservoirs in coastal area, indicating the critical role of recirculating systems in the terms of ARGs pollution control.

https://www.sciencedirect.com/science/article/pii/S0960852418300427