发布者:抗性基因网 时间:2018-04-03 浏览量:1459
摘要
在人类和动物药物中使用抗生素已经对抗生素耐药菌的全球传播施加了选择性压力。因此,制定策略来缓解农场,医院和流域等环境水库中的抗性基因的持续放大和传播是有意义的。然而,缓解策略的功效难以评估,因为尚不清楚哪些抗性基因对监测是重要的,以及使用哪种引物来检测这些基因。在这里,我们评估了一种类型的环境(肥料)中的一种大环内酯类抗生素抗性基因(erm)的多样性,以确定哪种引物最适合用于该环境的缓解研究。我们分析了所有已知的erm基因,并评估了先前发表的erm引物检测多样性的能力。结果显示所有已知的erm抗性基因分为66个簇,并且这些簇中的25个(40%)可以用文献中找到的引物靶向。这些引物可以靶向分析的粪肥中74%-85%的erm基因多样性。
Use of antibiotics in human and animal medicine has applied selective pressure for the global dissemination of antibiotic-resistant bacteria. Therefore, it is of interest to develop strategies to mitigate the continued amplification and transmission of resistance genes in environmental reservoirs such as farms, hospitals and watersheds. However, the efficacy of mitigation strategies is difficult to evaluate because it is unclear which resistance genes are important to monitor, and which primers to use to detect those genes. Here, we evaluated the diversity of one type of macrolide antibiotic resistance gene (erm) in one type of environment (manure) to determine which primers would be most informative to use in a mitigation study of that environment. We analyzed all known erm genes and assessed the ability of previously published erm primers to detect the diversity. The results showed that all known erm resistance genes group into 66 clusters, and 25 of these clusters (40%) can be targeted with primers found in the literature. These primers can target 74%–85% of the ermgene diversity in the manures analyzed.
https://academic.oup.com/femsec/article/94/4/fiy006/4810543