摘要

这项研究的目的是调查九个潜在的指示性抗菌素抗性基因（ARGs）（sul1，sul2，tetB，tetM，ermB，ermF，fexA，cfr，intI1）的命运和细菌群落对禽类粪便反应的多样性在90天内施用于可耕土壤。使用16S rDNA基因的定量实时PCR和Illumina高通量测序来量化和追踪ARG命运。随着时间的推移，所有基因的水平显着降低，并且intI1，sul1，sul2和tetM总是具有最大的丰度和最低的消散率。这表明应该更多的努力侧重于从粪肥中消除ARG，而不是等待环境中随后的衰减。我们的测序结果记录了这些实验中微生物群落结构和多样性的显着变化。在家禽粪肥组中，拟杆菌和放线菌是两个主要的门，而酸杆菌占主导地位的控制组。此外，含有潜在机会病原体的棒状杆菌属，假单胞菌属，赭曲霉属，马氏放线菌属和芽孢杆菌属的相对丰度随时间而改变，这表明家禽粪便不仅强烈影响细菌群落组成，而且选择特定的细菌群落。这项研究提供了施用家禽粪便后土壤中ARG命运和细菌群落多样性的一瞥。

The aim of this study was to investigate the fate of nine potential indicator antimicrobial resistance genes (ARGs) (sul1, sul2, tetB, tetM, ermB, ermF, fexA, cfr, intI1) and the diversity of bacterial communities in response to poultry manure applications to arable soil over a 90 day period. Quantitative real time PCR and Illumina high-throughput sequencing of 16S rDNA gene were used to quantify and trace ARG fate. The levels of all genes dramatically decreased over time and intI1, sul1, sul2 and tetM always had the greatest abundance and lowest dissipation rates. This indicated that more effort should be focused on the ARG elimination from manure rather than waiting for subsequent attenuation in the environment. Our sequencing results documented dramatic changes in the microbial community structure and diversity during these experiments. In poultry manure groups,Bacteroidetes and Actinobacteria were the two dominant phyla while Acidobacteriadominated the control groups. Moreover, the relative abundance of generaCorynebacterium, Pseudomonas, Ochrobactrum, Actinomadura and Bacillus, which contained potential opportunistic pathogens, changed over time suggesting that poultry manure not only strongly influenced bacterial community composition, but also selected specific bacterial communities. This study provides a glimpse of ARG fates and bacterial community diversity in soil after the application of poultry manure.

https://www.sciencedirect.com/science/article/pii/S014765131730653X