摘要

分布在酒店，旅馆和宾馆等住宅设施中的水一般用于饮用和沐浴等多种用途。它不是无菌的，不管是否应用了水处理。管道水中微生物的存在导致配送系统基础设施和生物膜形成的殖民化。生物膜是结构良好的多细胞群落，它们埋藏在自发产生的超聚合物质中，作为抗生素扩散的障碍。生物膜中的细菌与其自由浮动细菌相比具有一些优势，其中包括防止消毒剂和抗大多数抗生素的能力，特别是在水管系统中。本研究的目的是调查分离自水龙头的生物膜中的抗生素抗性及其抗性基因，其将饮用水家庭中的最终用户给水。从6个招待所收集了36个生物膜样本。在R2A培养基上使用扩散平板法分离总共108个生物膜细菌。使用纸片扩散法测定抗生素敏感性试验。用16S rRNA基因测序和3个抗性基因鉴定分离物;通过聚合酶链式反应检测tetA，tetM和ermB。产碱杆菌，蜡状芽孢杆菌，肠杆菌属，梭状芽孢杆菌，富梗病甲氧球菌，铜绿假单胞菌，普罗威登斯菌和液化沙雷氏菌。 Alcaligenes spp是所有Hospitality家庭中最频繁分离的。 tetA，抗性基因更为普遍。在所有生物膜样品中49％的生物膜分离物中检测到tetM，45％和ermB，46％。从生物膜分离的细菌对氯霉素高度耐药（100％），而对亚胺培南的耐药性最低（1％）。鉴于以上所述，因此迫切需要酒店管理部门制定出消除与酒店住宅相关的生物膜细菌的方式，由于含有抗生素抗性基因的多药耐药性细菌的存在，这可能构成巨大的公共健康风险。

Water distributed in residential facilities such as Hotels, Inns and Guest houses are generally intended for several purposes like drinking and bathing. It is not sterile, regardless the water treatment been applied. Microbial presence in pipe-borne water results in the colonization of the distribution systems infrastructure and biofilm formation. Biofilms are well structured multicellular communities which are buried in a self-produced extra-polymeric substance that functions as an obstacle to antibiotic diffusion. Bacteria in biofilms have some advantage over their free floating counterparts which include protection from disinfectants and ability to resist most antibiotics especially in water piped systems. The aim of this study was to investigate the antibiotic resistance and their resistance genes in biofilm isolated from faucets giving water to end users in hospitality homes. Thirty six biofilm samples were collected from 6 hospitality homes. A total of 108 biofilms bacteria were isolated using spread plate method on R2A medium. Antibiotic susceptibility test was determined using disk diffusion method. Isolates were characterized using 16S rRNA gene sequencing and three resistance genes; tetA, tetM and ermB were detected by Polymerase chain reaction. Alcaligenes faecalis, Bacillus cereus, Enterobacter sp, Lysinibacillus fusiformis,Methylobacterium fujisawaense, Pseudomonas aeruginosa, Providencia vermicola, and Serratia liquefaciens, were isolated. Alcaligenes spp was the most frequently isolated in all Hospitality homes. tetA, resistance gene was more prevalent. It was detected in 49% of biofilm isolates, tetM, 45%, and ermB, 46% of all biofilm samples. Bacteria isolated from Biofilm were highly resistant to Chloramphenicol (100%) while lowest resistance was to Imipenem (1%). In view of the above, there is therefore an urgent need for the Hotel Management to work out modalities in eliminating biofilm bacteria associated with hospitality homes, which could pose a great public health risk due to the presence of multidrug resistant bacteria harboring antibiotic resistant genes.

http://ijoehy.it/index.php/IJOEHY/article/view/252