摘要

抗微生物药物耐药性（AMR）是一个全球性的健康问题。为了尽量减少对宇航员的这种威胁，这些宇航员可能会免疫受损，从而导致感染抗微生物药物的病原体的风险更大，国际空间站“抗体组”的综合研究已经进行。使用全基因组测序（WGS）和盘扩散抗生素抗性测定法，从ISS分离的9个生物安全2级生物被评估其抗生素抗性。用Ion AmpliSeq™和宏基因组学分析来自ISS收集的24个表面样品的AMR基因的分子分析，所述样品在一年中的3个不同取样事件期间进行分析。盘旋扩散测定显示肠杆菌bugandensis菌株对所有测试的9种抗生素都有抗性，而溶血葡萄球菌对任何抗生素均无抗性。 Ion AmpliSeq™揭示发现了123个AMR基因，其中负责β-内酰胺和甲氧苄啶抗性的基因是最丰富和最普遍的。使用多种方法，首次从ISS检查了参与抗微生物药物耐药性的基因。这些信息可能会导致缓解战略，以便在长期太空任务期间保持宇航员的健康，因为不可能返回地球接受治疗。

Antimicrobial resistance (AMR) is a global health issue. In an effort to minimize this threat to astronauts, who may be immunocompromised and thus at a greater risk of infection from antimicrobial resistant pathogens, a comprehensive study of the ISS “resistome’ was conducted. Using whole genome sequencing (WGS) and disc diffusion antibiotic resistance assays, 9 biosafety level 2 organisms isolated from the ISS were assessed for their antibiotic resistance. Molecular analysis of AMR genes from 24 surface samples collected from the ISS during 3 different sampling events over a span of a year were analyzed with Ion AmpliSeq™ and metagenomics. Disc diffusion assays showed thatEnterobacter bugandensis strains were resistant to all 9 antibiotics tested and Staphylococcus haemolyticus being resistant to none. Ion AmpliSeq™ revealed that 123 AMR genes were found, with those responsible for beta-lactam and trimethoprim resistance being the most abundant and widespread. Using a variety of methods, the genes involved in antimicrobial resistance have been examined for the first time from the ISS. This information could lead to mitigation strategies to maintain astronaut health during long duration space missions when return to Earth for treatment is not possible.

https://f.glgooo.top/scholar?start=50&q=antibiotic+resistance+genes&hl=zh-CN&as_sdt=0,5&as_ylo=2018