摘要

先前报道了从肉中分离的多种凝固酶阴性葡萄球菌（CNS）物种，并通过获得来自其他细菌的水平转移元件（例如毒性，抗生素抗性因子）而被认为是潜在的致病葡萄球菌。在这项研究中，研究了从零售市场180个生鸡肉样品中分离出的中枢神经系统的流行率，同源性和致病性。不同来源（包括不同的零售环境，供应商和产品）中，CNS总体患病率为11.7％，集中在表皮葡萄球菌69.2％和葡萄球菌warneri 30.8％。分子分型在同一物种中显示高度同源的模式。抗生素药敏试验显示，所有中枢神经系统都存在常见的青霉素和红霉素耐药，而17株表皮葡萄球菌分离株对庆大霉素（GM） - 卡那霉素（K） - 青霉素（P） - 红霉素（E）表现出多重耐药性，除一种耐甲氧西林在葡萄球菌盒式染色体mec（SCCmec）中分型的表皮葡萄球菌分离株Ⅴ。总之，多样化的生鸡肉原料产品展示了表皮葡萄球菌和S. warneri的集中流行，并且在相同的CNS种类中具有高度同质的基因组。虽然中枢神经系统产生了负面的毒性作用，但表皮葡萄球菌中的多重耐药性和中枢神经系统中的P-E耐药性需要高度关注人体健康。

Diverse coagulase-negative staphylococci (CNS) species isolated from meat are previously reported and considered as potential pathogenic staphylococci through obtain horizontal transferred elements (e.g. toxic, antibiotic resistance factors) from other bacteria. In this study, prevalence, homology and pathogenicity of CNS isolated from 180 raw chicken meat samples in retail markets were studied. The overall prevalence of CNS was 11.7% and centralized in 69.2% of Staphylococcus epidermidis and 30.8% of Staphylococcus warneri among diverse sources (including different retail settings, suppliers and products). Molecular typing showed highly homogenous patterns in the same species. Antibiotic susceptibility test revealed a common penicillin and erythromycin resistance among all of CNS, while 17 S. epidermidis isolates displayed multidrug resistance of gentamycin (GM)-kanamycin (K)-penicillin (P)-erythromycin (E), except one methicillin-resistant S. epidermidis isolate which was typed in staphylococcal cassette chromosome mec (SCCmec) Ⅴ. Conclusively, diverse sourced raw chicken meat products exhibited a centralized prevalence of S. epidermidis and S. warneri, and a highly homogenous of genome in the same CNS specie. Though the negative toxigenicity of CNS was determined, constant multidrug resistance in S. epidermidis and P-E resistance in CNS need to be highly concerned regarding human health.

https://www.sciencedirect.com/science/article/pii/S0023643817309246