摘要

万古霉素耐药肠球菌( VRE )已在野生动物中检测到，这代表了一个公共健康问题。红腿鹧鸪是一种常见的野鸟，其肉在包括葡萄牙在内的几个国家被食用。对来自葡萄牙北部的350份红腿鹧鸪粪便样品进行了VRE筛查。样品在添加万古霉素( 4 mg / L )的斯莱特兹-巴特利琼脂上培养，回收6株屎肠球菌。对分离株进行抗生素抗性和毒力基因检测。采用多位点序列分型( MLST )研究了含钒屎豆的基因型多样性。6株菌株均表现为红霉素抗性，并含有erm ( B )基因，4株菌株表现为四环素抗性。对氨苄青霉素耐药株pbp5基因的C末端区(最小抑制浓度范围为256μg / ml )进行了测序。考虑到461～629区氨基酸的变化，检测到两个不同的pbp5等位基因。所有分离株均含有esp基因，而hyl与esp基因一起在5个分离株中检测到。MLST分析将分离物分组为ST448 ( n = 1 )、ST139 ( n = 1 )和ST18 ( n = 4 )。结果表明，红腿鹧鸪可能是一个抗微生物基因库，可能有助于抗微生物基因向其他动物和人类的传播和转移。

Vancomycin-resistant enterococci (VRE) have been detected in wild animals representing a public health concern. The red-legged partridge (Alectoris rufa) is a common game bird and its meat is consumed in several countries, including Portugal. Three hundred five fecal samples of red-legged partridge from the north of Portugal were screened for VRE. Samples were cultured on Slanetz-Bartley agar supplemented with vancomycin (4 mg/L) and six vanA-Enterococcus faecium were recovered. Isolates were tested for antibiotic resistance and virulence genes. Multilocus sequence typing (MLST) was performed to study the genotypic diversity of vanA-containing E. faecium. The six isolates showed erythromycin resistance and harbored the erm(B) gene and the four that were tetracycline resistant showed the tet(M) gene. The C-terminal region of the pbp5 gene of the ampicillin-resistant isolates (minimal inhibitory concentration range of 256 μg/ml) was sequenced. Two different pbp5 alleles were detected when considering the changes of amino acid in 461–629 region. All isolates harbored the esp gene, whereas hyl, together with the esp gene, was detected in five isolates. MLST analysis grouped the isolates as ST448 (n = 1), ST139 (n = 1), and ST18 (n = 4). Our findings show that the red-legged partridges could be a reservoir of antimicrobial resistance genes and may contribute to the dissemination and transference of the resistance genes to other animals and humans.

https://www.liebertpub.com/doi/abs/10.1089/mdr.2017.0040