摘要

本研究合成了一种新型Au-graphene oxide（GO）-Co3O4空心球（Au / GO-Co3O4），并研究了其与抗生素抗性基因（ARGs）结合并抑制其复制的潜力。由于在环境中高度检测到具有两个扩增子长度的四环素抗性基因（tetA）作为模型ARG。 Au / GO-Co3O4复合材料是用四正丁基溴化铵（TBAB）作为中介代用品合成的。我们发现它可以与tetA结合，并因此与纯Co3O4和GO-Co3O4杂交体相比更大程度地抑制其复制。此外，Au-GO-Co3O4复合物与长片段（L-tetA）结合的短tetA-ARG片段（S-tetA）更有效。利用光谱方法（FTIR，Raman，UV-Vis和荧光光谱），分离滤液分析和凝胶电泳系统分析复合物与tetA之间的相互作用机制。发现GO和金纳米颗粒对tetA的吸附以及钴氧化物/钴离子的裂解作用在tetA的捕获和破坏中起关键作用。此外，我们发现Co3O4和GO-Co3O4与tetA的相互作用主要是通过插层机制，然而，带有tetA的Au / GO-Co3O4主要通过沟槽结合机制。本研究中的这些发现揭示了用新型抑制剂净化ARGs污染水和消除ARG抗生素耐药性的新方法。

A novel Au-graphene oxide (GO)-Co3O4 hollow sphere (Au/GO-Co3O4) was synthesized and its potential in binding with antibiotic resistance genes (ARGs) and inhibiting their replication was examined in this study. Tetracycline-resistant genes (tetA) with two amplicon lengths were selected as the model ARGs due to its highly detected in the environment. The Au/GO-Co3O4 composite was synthesized using tetra-n-butylammonium bromide (TBAB) as a mediating proxy. We found that it could bind withtetA and consequently inhibit its replication to a greater extent compared with pure Co3O4 and GO-Co3O4 hybrid. Furthermore, the Au-GO-Co3O4 composite was more efficient in binding with short fragments of tetA-ARGs (S-tetA) than long fragments (L-tetA). The interaction mechanism between the composite and tetA was systematically analyzed using spectra methods (FTIR, Raman, UV–Vis, and fluorimetric spectra), isolated filtrate assays, and gel electrophoresis. It is found that the adsorption of both GO and gold nanoparticles for tetA as well as cleavage effect of cobalt oxide/cobalt ions played a critical role in the capture and damage of tetA. Furthermore, we found that the interaction of both Co3O4 and GO-Co3O4 with tetA was mainly through intercalation mechanism, however, that of Au/GO-Co3O4 with tetA was mainly through groove binding mechanism. These findings in this study throw light on the new approaches for purification of ARGs contaminated water and elimination of antibiotic resistance of ARGs by novel inhibitor.

https://www.sciencedirect.com/science/article/pii/S1385894718304662