摘要

使用全基因组测序来检查持续的屎肠球菌菌血症，其获得对三种抗生素的异源性以响应延长的多药物疗法。 每次改变前后的完整基因组的比较揭示了万古霉素和利奈唑胺的已知耐药决定簇的出现，并且表明编码脂肪酸合酶的fabF中的新突变负责达托霉素非易感性。 撤回药物后，质粒重组有助于万古霉素耐药性的进行性丧失。

Whole-genome sequencing was used to examine a persistent E. faeciumbacteremia that acquired heteroresistance to three antibiotics in response to prolonged multidrug therapy. Comparison of complete genomes before and after each change revealed the emergence of known resistance determinants for vancomycin and linezolid, and suggested that a novel mutation in fabF, encoding a fatty acid synthase, was responsible for daptomycin nonsusceptibility. Plasmid recombination contributed to progressive loss of vancomycin resistance after withdrawal of the drug.

http://aac.asm.org/content/early/2018/01/09/AAC.02007-17.abstract