摘要

耐甲氧西林葡萄球菌（MRS）是公众和动物健康的主要关注点。对30种MRS（金黄色葡萄球菌，S. cohnii，表皮葡萄球菌，溶血葡萄球菌，S. hominis，S. lentus，使用标准琼脂扩散法和市售小型化系统（Sensi试验革兰氏阳性）对11种抗菌剂（属于7种不同类别）的临床意义。值得强调的是，16株（53.33％）表现出广泛的耐药表型（XDR）。每个菌株的平均抗性数是4.67。这些结果表明零售肉类和家禽制品可能是多药耐药MRS传播的载体。红霉素耐药率最高（76.67％），其次是妥布霉素，头孢他啶（66.67％），环丙沙星（56.67％）和磷霉素（53.33％）。两种测试方法之间的一致性（卡帕系数）为0.64。以琼脂扩散片为参考方法，小型化试验的敏感性，特异性和准确性分别为98.44％，69.44％和83.33％。两种方法之间的差异大部分是由于根据椎间盘扩散法敏感的分离物，但是根据微型化试验（假阳性）耐受。

Methicillin-resistant staphylococci (MRS) are a major concern to public and animal health. Thirty MRS (Staphylococcus aureus, S. cohnii, S. epidermidis, S. haemolyticus, S. hominis, S. lentus, S. lugdunensis, S. sciuri, and S. xylosus) isolates from meat and poultry preparations were tested for antimicrobial susceptibility to 11 antimicrobials (belonging to seven different categories) of clinical significance using both the standard agar disc diffusion method and a commercially available miniaturized system (Sensi Test Gram-positive). It is worth stressing that 16 isolates (53.33%) exhibited an extensively drug-resistant phenotype (XDR). The average number of resistances per strain was 4.67. These results suggest that retail meat and poultry preparations are a likely vehicle for the transmission of multi-drug resistant MRS. Resistance to erythromycin was the commonest finding (76.67% of strains), followed by tobramycin, ceftazidime (66.67%), ciprofloxacin (56.67%) and fosfomycin (53.33%). An agreement (kappa coefficient) of 0.64 was found between the two testing methods. Using the agar disc diffusion as the reference method, the sensitivity, specificity and accuracy of the miniaturized test were 98.44%, 69.44% and 83.33%, respectively. Most discrepancies between the two methods were due to isolates that were susceptible according to the disc diffusion method but resistant according to the miniaturized test (false positives).

https://www.sciencedirect.com/science/article/pii/S0740002017302708