摘要

尽管与人类报道的沙门氏菌病不频繁相关，但沙门氏菌肠炎亚种。 enterica serovar肯塔基州（ser。Kentucky）是美国报道的最常见的非临床非人类血清型。这项研究的目标是使用群集规则间隔短回文重复序列（CRISPR） - 多毒力 - 基因座序列分型（MVLST）亚型人类临床分离株的集合。肯塔基州向宾夕法尼亚州卫生部提交并确定这些菌株中抗生素耐药性的程度。这一分析突出了ser的多重性质。肯塔基州，并将我们的菌株分为两组，我们的组合中有相同的代表组别I和组II。此外，使用抗生素国家抗生素耐药监测系统（NARMS）对所有菌株进行抗生素药敏试验表明，耐药谱可分为两组。第一组分离株对头孢菌素和青霉素耐药，而第二组分离菌对喹诺酮，庆大霉素和磺胺异恶唑耐药。总体而言，50％的分离株对三种或更多类抗生素有抗性，30％对五种或更多种抗药性有抗性。抗生素耐药性与两种不同谱系的相关性可能反映了两个不同血清储库内的适应性。肯塔基州，与抗微生物剂有差别暴露。

Although infrequently associated with reported salmonellosis in humans, Salmonella enterica, subsp. enterica serovar Kentucky (ser. Kentucky) is the most common nonclinical, nonhuman serovar reported in the United States. The goal of this study was to use Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–multi-virulence-locus sequence typing (MVLST) to subtype a collection of human clinical isolates of ser. Kentucky submitted to the Pennsylvania Department of Health and to determine the extent of antibiotic resistance in these strains. This analysis highlighted the polyphyletic nature of ser. Kentucky, and separated our isolates into two groups, Group I and Group II, which were equally represented in our collection. Furthermore, antimicrobial susceptibility testing on all isolates using a National Antimicrobial Resistance Monitoring System (NARMS) panel of antibiotics demonstrated that resistance profiles could be divided into two groups. Group I isolates were resistant to cephems and penicillins, whereas Group II isolates were resistant to quinolones, gentamicin, and sulfisoxazole. Collectively, 50% of isolates were resistant to three or more classes of antibiotics and 30% were resistant to five or more classes. The correlation of antibiotic resistance with the two different lineages may reflect adaptation within two distinct reservoirs of ser. Kentucky, with differential exposure to antimicrobials.

https://www.liebertpub.com/doi/abs/10.1089/fpd.2017.2298