摘要
      在中试规模上研究了低压膜过滤对传统二级处理废水中抗微生物基因（ARGs）的去除。在操作微滤（MF）和超滤（UF）膜时，检查了抗微生物耐药性（AMR）研究的关键操作参数以及影响低压膜过滤过程中抗微生物耐药性去除效率的关键因素。去除AMR的主要因素是膜的孔径。毛细管膜上污垢层的形成对染色体内和染色体外ARG的去除只有很小的相加作用，而对移动ARG的清除有显著的相加作用。使用具有不同ARG丰度的饲料表明，饲料中ARG丰度越高，滤液中的ARG丰度就越高。UF滤液中的活死细胞计数显示完整的细菌突破UF膜。超滤滤液中16S rRNA基因（作为细菌定量的替代物）和sul1基因之间的强相关性表明ARBs可能会突破超滤膜。
Abstract
Low-pressure membrane filtration was investigated at pilot scale with regard to its removal of antimicrobial resistance genes (ARGs) in conventional secondary treated wastewater plant effluents. While operating microfiltration (MF) and ultrafiltration (UF) membranes, key operational parameters for antimicrobial resistance (AMR) studies and key factors influencing AMR removal efficiencies of low-pressure membrane filtration processes were examined. The main factor for AMR removal was the pore size of the membrane. The formation of the fouling layer on capillary membranes had only a small additive effect on intra- and extrachromosomal ARG removal and a significant additive effect on mobile ARG removal. Using feeds with different ARGs abundances revealed that higher ARG abundance in the feed resulted in higher ARG abundance in the filtrate. Live-Dead cell counting in UF filtrate showed intact bacteria breaking through the UF membrane. Strong correlations between 16S rRNA genes (as surrogate for bacteria quantification) and the sul1 gene in UF filtrate indicated ARBs likely breaking through UF membranes.

https://www.sciencedirect.com/science/article/abs/pii/S0048969722031497