摘要
      在这项研究中，我们使用基于高通量测序的宏基因组方法，研究了越南宁平Day河下游虾池和河水样本中细菌群落的季节结构以及抗生素抗性基因（ARGs）和移动遗传元件（MGE）的丰度和多样性的差异。中心细菌群落、ARGs和MGE的结构被发现与季节和地点无关。在所有样本中发现的主要门是变形菌门、拟杆菌门和放线菌门。多药耐药（MDR）基因和转座酶分别是最主要的ARG类型和MGE。我们的数据显示，雨季河水中细菌群落、ARGs和MGE的丰度更高。ARGs、MGE的丰度与环境因素之间存在显著相关性。我们的研究结果表明，含有携带ARGs/MGEs细菌的水环境对虾和人类健康构成风险，尤其是在降雨污染的水季节。
Abstract
In this study, we use high-throughput sequencing-based metagenomic methods to investigate the differences in seasonal structures of the bacterial community and the abundance and diversity of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in both shrimp ponds and river water samples downstream of the Day River, Ninh Binh, Vietnam. The structure of the central bacterial community, ARGs, and MGEs was found to be regardless of the seasons and locations. The predominant phyla found in all samples was Proteobacteria, Bacteroidetes, and Actinobacteria. Multi-drug resistance (MDR) genes and transposases are the most dominant ARG types and MGEs, respectively. Our data showed a higher abundance of bacterial communities, ARGs, and MGEs in the river water during the rainy season. There is a significant correlation between the abundance of ARGs, MGEs, and environmental factors. Our results indicate that water environments containing ARGs/MGEs carrying bacteria pose a risk to shrimp and human health, especially during the rainfall-polluted water season.

https://link.springer.com/article/10.1186/s13765-022-00687-w