摘要
      本研究的目的是利用两种不同的生物炭（椰子壳CSB和竹生物炭BB）作为改良剂，研究猪粪（PM）堆肥中的抗生素抗性真菌（ARF）群落。设计了三种处理（对照、10%CSB和10%BB），并以T1至T3指示。实验结果表明，在两种生物炭处理中，真菌丰度显著降低，但三个优势门子囊菌门、担子菌门和毛霉菌门的丰度仍然相对较高。三种处理之间真菌的相对丰度和多样性均存在显著差异（p<0.05）。有趣的是，添加生物炭可以调节最终堆肥中的整体真菌群落。与对照组相比，真菌的丰度得到了积极调动，尤其是CSB表现出更好的效果。总之，生物炭有可能抑制和减少堆肥中ARGs的数量和流动性。因此，这些发现为理解PM堆肥过程中ARF的连续性提供了新的见解。
Abstract
The purpose of this study was to investigate antibiotic resistant fungal (ARF) communities in pig manure (PM) composting employing two different biochar (coconut shell-CSB and bamboo biochar-BB) as amendment. Three treatments (Control, 10% CSB and 10% BB) were designed and indicated with T1 to T3. Experimental results declared that the fungal abundance significantly reduced among the both biochar applied treatments but three dominant phyla Ascomycota, Basidiomycota and Mucoromycota were still relatively greater abundance present. There were significant differences (p < 0.05) in the relative abundance and diversity of fungi among all three treatments. Interestingly, biochar addition regulated the overall fungal community in final compost. Compared with the control group, the abundance of fungi was positively mobilized, and especially CSB showed a better effect. Conclusively, biochar has potential to inhibit and reduce the ARGs population and mobility in compost. Thus, these findings offer new insight to understand the succession of ARFs during PM composting.

https://www.sciencedirect.com/science/article/abs/pii/S026974912201449X