摘要
      本研究旨在探讨环丙沙星（CIP，0.05–40 mg/L）和铜（3 mg/L）联合污染对应激期和效应后活性污泥系统硝化作用、微生物群落和抗生素抗性基因（ARGs）的影响。较高的CIP浓度抑制了硝化作用，在40mg/L的CIP压力下，总氮去除率平均为50%。应激和应激后对细菌多样性和结构的影响明显不同。根据全面分类，丰度属比稀有属对联合污染更敏感，条件稀有或丰度分类群是它们相互作用的关键分类群。氨氧化基因在高CIP水平下受到抑制，但一些好氧反硝化细菌（Thauera、Comamonas和Azoarcus）和关键基因增加。检测到96种ARG亚型，具有复杂的正相关关系，它们的潜在宿主（丰富的稀有功能属）在两个时期内发生了变化。这项研究强调了复合污染对活性污泥的不同应激和后效应。
Abstract
This study aimed to explore the impacts of ciprofloxacin (CIP, 0.05–40 mg/L) and copper (3 mg/L) combined pollution on nitrification, microbial community and antibiotic resistance genes (ARGs) in activated sludge system during stress- and post-effect periods. Higher CIP concentration inhibited nitrification and an average of 50% total nitrogen removal occurred under 40 mg/L of CIP pressure. The stress- and post-effects on bacterial diversity and structure were obviously distinct. Abundant genera were more sensitive to combined pollution than rare genera based on full-scale classification and conditionally rare or abundant taxa were keystone taxa in their interactions. Ammonia oxidation genes were inhibited under high CIP level, but some aerobic denitrifying bacteria (Thauera, Comamonas and Azoarcus) and key genes increased. 96 ARG subtypes were detected with complex positive relationships and their potential hosts (abundant-rare-functional genera) changed in two periods. This study highlights the different stress- and post-effects of combined pollution on activated sludge.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422002115