发布者:抗性基因网 时间:2023-06-07 浏览量:240
摘要
抗微生物耐药性(AMR)的迅速传播对全球健康构成威胁,一旦使用抗微生物剂,同时存在的抗微生物耐药性基因(ARGs)的性质可能会导致副作用。因此,确定哪些ARG对同时出现是至关重要的。鉴于公共存储库中有丰富的NGS数据,我们调查了214095个宏基因组数据集中ARG丰度之间的相关性。使用与ARGs比对的超过6.76·108个读取片段来推断成对相关系数,我们发现在人类和动物采样来源中相互关联的ARGs比在土壤和水环境中更多。此外,我们发现,这些相关性是对至关重要的抗菌药物同时发生耐药性的风险特征。使用这些图谱,我们发现了几个关键的ARGs,这些ARGs间接但强烈地选择了关键的ARG,例如与大多数形式的抗性相关的四环素ARGs。总之,这项研究强调了ARG参与者间接参与塑造各种环境的抵抗力,这些抵抗力可以作为AMR监测计划的监测目标。
Abstract
The rapid spread of antimicrobial resistance (AMR) is a threat to global health, and the nature of co-occurring antimicrobial resistance genes (ARGs) may cause collateral AMR effects once antimicrobial agents are used. Therefore, it is essential to identify which pairs of ARGs co-occur. Given the wealth of NGS data available in public repositories, we have investigated the correlation between ARG abundances in a collection of 214,095 metagenomic datasets. Using more than 6.76·108 read fragments aligned to ARGs to infer pairwise correlation coefficients, we found that more ARGs correlated with each other in human and animal sampling origins than in soil and water environments. Furthermore, we showed that the correlations serve as risk profiles of resistance co-occurring to critically important antimicrobials. Using these profiles, we found several key ARGs indirectly but strongly selecting for ARGs of critical importance, such as tetracycline ARGs correlating with most forms of resistances. In conclusion, this study highlights the important ARG players indirectly involved in shaping the resistomes of various environments that can serve as monitoring targets in AMR surveillance programs.
https://www.biorxiv.org/content/10.1101/2022.12.19.519133v1.abstract