摘要
      污水处理厂被认为是抗生素耐药性出现和扩散的“热点”。然而，重金属污染对STPs中抗生素耐药性扩散的影响尚不清楚。这项研究同时调查了印度德里不同STPs处理单元去除金属和抗生素耐药性以及可移动元素的效果。结果表明，STPs中使用的处理技术在完全消除金属和抗生素耐药性方面效率低下，存在抗生素耐药性共同选择的生态风险。在重金属、金属和抗生素耐药性以及整合素之间观察到了强烈的相关性，这意味着在重金属存在的情况下，抗生素耐药性可能会通过整合素而加剧，并且金属和抗生素耐药具有共同或密切相关的机制。我们量化了一种MRG rcnA，赋予了对Co和Ni的耐药性，并确定它比所有MRG、ARGs、整合子和16S rRNA都更丰富，这表明rcnA在环境中抗生素耐药性传播中可能很重要。重金属、金属和抗生素耐药性以及整合子之间的关联突出表明，需要进行更多的研究，以更好地理解共同选择的机制，并提高当前治疗系统的去除效果。
Abstract
Sewage treatment plants (STPs) are considered as “hotspots” for the emergence and proliferation of antibiotic resistance. However, the impact of heavy metals contamination on dispersal of antibiotic resistance in STPs is poorly understood. This study simultaneously investigated the effect of removal of metal and antibiotic resistance as well as mobile elements at different treatment units of STPs in Delhi, India. Results showed that treatment technologies used in STPs were inefficient for the complete removal of metal and antibiotic resistance, posing an ecological risk of co-selection of antibiotic resistance. The strong correlations were observed between heavy metals, metal and antibiotic resistance, and integrons, implying that antibiotic resistance may be exacerbated in the presence of heavy metals via integrons, and that metal and antibiotic resistance share a common or closely associated mechanism. We quantified an MRG rcnA, conferring resistance to Co and Ni, and identified that it was more abundant than all MRGs, ARGs, integrons, and 16S rRNA, suggesting rcnA could be important in antibiotic resistance dissemination in the environment. The associations between heavy metals, metal and antibiotic resistance, and integrons highlight the need for additional research to better understand the mechanism of co-selection as well as to improve the removal efficacy of current treatment systems.

https://www.sciencedirect.com/science/article/abs/pii/S0269749122008636