摘要
      土壤微生物燃料电池已被应用于单一抗生素污染土壤的原位修复。然而，仅对单一抗生素污染的调查阻碍了MFC在现实世  界土壤修复中的应用，在现实世界中，具有相似化学结构的多种抗生素对抗生素及其相应的抗生素抗性基因（ARGs）命运的影响仍然未知。在本研究中，通过添加两种常用抗生素（磺胺嘧啶，SDZ和磺胺甲恶唑，SMX），并将其与仅添加单一抗生素（SDZ）进行比较，研究了土壤MFC中抗生素的去除率、微生物群落组成、代谢产物组成和ARG丰度。与单一抗生素相比，添加混合抗生素的土壤MFC中的抗生素去除率更高，这是因为抗生素污染土壤的上层（初始抗生素浓度的57.24%）和下层（初始浓度的57.07%）的生物降解效率都提高了。混合抗生素条件下的细菌群落多样性增加，这可能是由于抗生素生物降解过程中产生的中间体毒性降低所致。此外，混合抗生素的添加降低了ARG释放到土壤环境中的风险，这反映在单一抗生素处理中宿主细菌的丰度更高。这些结果鼓励了土壤MFC技术的进一步发展，用于抗生素污染土壤的原位修复。
Abstract
Soil microbial fuel cells (MFCs) have been applied for the in situ remediation of soils polluted by single antibiotics. However, the investigation of only single antibiotic pollution has hindered MFC application in real-world soil remediation, where the effects of multiple antibiotics with similar chemical structures on the fate of antibiotics and their corresponding antibiotic resistance genes (ARGs) remain unknown. In this study, antibiotic removal rates, microbial community compositions, metabolite compositions, and ARG abundances were investigated in soil MFCs by adding two commonly used antibiotics (sulfadiazine, SDZ, and sulfamethoxazole, SMX), and comparing them with the addition of only a single antibiotic (SDZ). The antibiotic removal rate was higher in the soil MFC with addition of mixed antibiotics compared to the single antibiotic due to enhanced biodegradation efficiency in both the upper (57.24% of the initial antibiotic concentration) and lower layers (57.07% of the initial concentration) of the antibiotic-polluted soils. Bacterial community diversity in the mixed antibiotic conditions increased, and this likely resulted from the decreased toxicity of intermediates produced during antibiotic biodegradation. Moreover, the addition of mixed antibiotics led to lower risks of ARG release into soil environments, as reflected by higher abundances of host bacteria in the single antibiotic treatment. These results encourage the further development of soil MFC technology for in situ remediation of antibiotic-polluted soils.

https://www.sciencedirect.com/science/article/abs/pii/S0048969722014991