摘要
      抗生素耐药性基因（ARGs）因其潜在的耐药性风险而被认为是新出现的污染物。水平基因转移（HGT）是ARGs导致环境威胁的主要途径。因此，ARGs的HGT的存在可以有效抑制单个菌株内ARGs潜在的耐药性风险。本文通过ARGs的HGT特征鉴定、转移机理分析和转移过程调控相结合，对水稻和旱地土壤中ARGs HGT的特征进行了鉴定和调控。使用同源性建模算法模拟大肠杆菌Tn5质粒转座酶的构建，以鉴定ARGs的HGT特性。因此，GCG（212.617Å）被确定为靶密码子。通过计算机综合方法，结果表明，水稻和旱地土壤中ARGs HGT最重要的环境干扰因素分别是粗糙的农家肥/污水灌溉和采矿污染。在关键环境因素的干扰下，水稻和旱地土壤中ARGs的HGT抑制作用分别降低了35.01%和34.74%。结果表明，所提出的理论机制和控制策略可以有效抑制土壤环境中大肠杆菌ARGs的HGT。
Abstract
Antibiotic resistance genes (ARGs) have been regarded as emerging pollutants due to their potential risk of resistance. Horizontal gene transfer (HGT) is the main pathway for ARGs to lead to environmental threats. Therefore, the inhabitation of ARGs' HGT can effectively inhibit ARGs' potential drug resistance risk within a single strain. In this paper, the characteristics of ARGs' HGT in paddy and dryland soils were identified and regulated by a combination of ARGs' HGT feature identification, transfer mechanism analysis and transfer process regulation. The homology modeling algorithm was used to simulate the construction of the Tn5 plasmid transposase of Escherichia coli (E. coli) for identifying ARGs' HGT characteristics. The GCG (212.617 Å) was thus determined as the target codon. Through integrated computer-based methods, results showed that the most important environmental disturbance factors for the HGT of ARGs in the paddy and dryland soils were rough farmyard manure/sewage irrigation and mining pollution, respectively. Under the disturbance of key environmental factors, the inhibitory effect of HGT of ARGs in paddy and dryland soil was reduced by 35.01 % and 34.74 %, respectively. Results demonstrated that the proposed theoretical mechanism and control strategies could effectively inhibit the HGT of E. coli ARGs in the soil environment.

https://www.sciencedirect.com/science/article/abs/pii/S0048969722061952