摘要
       零价铁（ZVI）激活的类芬顿工艺已被提出用于灭活废水中携带不同抗生素抗性基因（ARG）的ARB，而它们降解携带ARG的ARB的能力非常低。我们之前的研究表明，预酸化可以使细胞失活和裂解。因此，本研究调查了预酸化对多药耐药细菌恶臭假单胞菌MX-2的灭活以及ZVI/H2O2和ZVI/PDS过程同时降解携带的七种ARGs的影响。结果表明，在ZVI/H2O2和ZVI/过氧二硫酸盐（PDS）工艺中，细菌溶液预酸化至酸性pH极大地促进了携带ARGs的降解，从0.14至0.17 log降解至2.48至2.97 log，同时几乎完全灭活了P.putida MX-2。此外，预酸化严重裂解了细菌细胞，将携带的ARGs释放到细胞外环境中，并促进了产生的自由基对细胞外ARGs（e-ARGs）的降解。在所得e-ARGs的降解过程中，它们通过ZVI/H2O2和ZVI/PDS过程被片段化为短片段。对含有P.putida MX-2的真实废水的处理进一步验证了预酸化对携带ARGs降解的促进作用。因此，通过酸化或其他方法有效裂解ARB细胞，极大地促进携带的ARG的降解，这一点极为关键，也可适用于其他先进的氧化工艺，有效降低废水中的抗生素耐药性。
Abstract
Zero-valent iron (ZVI) activated Fenton-like processes have been proposed to inactivate ARB that carry diverse antibiotic resistance genes (ARGs) in wastewater, whereas they exhibit very low capacity of degrading ARB-carrying ARGs. Our previous study has revealed that pre-acidification can make cell inactivation and lysis. Thus, the present study investigated the effect of pre-acidification on the inactivation of a multi-drug resistant bacterium Pseudomonas putida MX-2 and the simultaneous degradation of carried seven ARGs by ZVI/H2O2 and ZVI/PDS processes. Results showed that the pre-acidification of bacterial solution to acidic pH greatly promoted the degradation of carried ARGs from 0.14 to 0.17 logs to 2.48–2.97 logs at the meanwhile of almost completely inactivating P. putida MX-2 in both ZVI/H2O2 and ZVI/peroxydisulfate (PDS) processes. Moreover, the pre-acidification seriously lysed the bacterial cells, released the carried ARGs into the extracellular environment, and facilitated the degradation of extracellular ARGs (e-ARGs) by the generated radicals. During the degradation of the resultant e-ARGs, they were fragmented to short fragments by ZVI/H2O2 and ZVI/PDS processes. The treatment of real wastewater containing P. putida MX-2 further validated the promoting effect of pre-acidification on the degradation of carried ARGs. Therefore, it is extremely crucial to lyse the cells of ARB efficiently via acidification or other methods to promote the degradation of carried ARGs greatly, which may also be applicable for other advanced oxidation processes to reduce antibiotic resistance in wastewater effectively.

https://www.sciencedirect.com/science/article/abs/pii/S1385894722048926